ArticleCommunications biology2021
Droplet-based microfluidic platform for high-throughput screening of Streptomyces.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 72 citations in OpenAlex.
- Construction of a library of gene expression elements in industrialSynthetic and systems biotechnology · 2026Article
- Selective access to individual cells after assembling for advanced cell-cell interaction studies.Journal of nanobiotechnology · 2026Article
- Precise loading of scarce reagents on droplet microarrays.bioRxiv : the preprint server for biology · 2026Article
- Genetically encoded biosensors enabled high-throughput screening of microbial cell factories.Engineering microbiology · 2026Review
- The effect of substrate and product transfer between polydisperse microdroplets on biological high-throughput screens.Scientific reports · 2026Article
- Synthetic Biology Strategies for Activating Cryptic BGCs inACS omega · 2026Review
- Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization inSynthetic and systems biotechnology · 2025Article
- Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review.Biotechnology letters · 2025Review
- An integrated platform for liquid handling and cell imaging in life science applications.Nature communications · 2025Article
- Size-independent and automated single-colony-resolution microdroplet dispensing.Lab on a chip · 2025Article
- Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application.Biosensors · 2025Review
- Emerging Diagnostics inInternational journal of molecular sciences · 2024Review
- Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system.World journal of microbiology & biotechnology · 2023Review
- High-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae.Biotechnology for biofuels and bioproducts · 2023Article
- Methyl halide transferase-based gas reporters for quantification of filamentous bacteria in microdroplet emulsions.Applied and environmental microbiology · 2023Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Droplet-Based Microfluidics: Applications in Pharmaceuticals.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Biosensors Based on Inorganic Composite Fluorescent Hydrogels.Nanomaterials (Basel, Switzerland) · 2023Review
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomyces are one of the most important industrial microorganisms for the production of proteins and small-molecule drugs. Previously reported flow cytometry-based screening methods can only screen spores or protoplasts released from mycelium, which do not represent the filamentous stationary phase Streptomyces used in industrial cultivation. Here we show a droplet-based microfluidic platform to facilitate more relevant, reliable and rapid screening of Streptomyces mycelium, and achieved an enrichment ratio of up to 334.2. Using this platform, we rapidly characterized a series of native and heterologous constitutive promoters in Streptomyces lividans 66 in droplets, and efficiently screened out a set of engineered promoter variants with desired strengths from two synthetic promoter libraries. We also successfully screened out several hyperproducers of cellulases from a random S. lividans 66 mutant library, which had 69.2-111.4% greater cellulase production than the wild type. Our method provides a fast, simple, and powerful solution for the industrial engineering and screening of Streptomyces in more industry-relevant conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.