ArticleHorticulture research2021
CRISPR/Cas9-mediated targeted mutation reveals a role for AN4 rather than DPL in regulating venation formation in the corolla tube of Petunia hybrida.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- The role of RaDFR2 regulating flower color formation in Rhododendron agastum.BMC plant biology · 2026Article
- Advancing ornamental plant breeding through genomic technologies: opportunities, challenges, and future directions.Functional & integrative genomics · 2025Review
- A chromosome-levelActa pharmaceutica Sinica. B · 2025Article
- Tissue-specific transcriptome analyses unveils candidate genes for flavonoid biosynthesis, regulation and transport in the medicinal plant Ilex asprella.Scientific reports · 2024Article
- Metabolite and Transcriptome Profiling Analysis Provides New Insights into the Distinctive Effects of Exogenous Melatonin on Flavonoids Biosynthesis inInternational journal of molecular sciences · 2024Article
- Transcriptome Analysis Reveals ThatCurrent issues in molecular biology · 2024Article
- The R2R3-MYB transcription factor ZeMYB32 negatively regulates anthocyanin biosynthesis in Zinnia elegans.Plant molecular biology · 2024Article
- Functional redundancy of R2R3-MYB transcription factors involved in anthocyanin biosynthesis is manifested in anther pigmentation in petunia.Plant biotechnology (Tokyo, Japan) · 2024Article
- Cell layer-specific expression of the homeotic MADS-box transcription factor PhDEF contributes to modular petal morphogenesis in petunia.The Plant cell · 2024Article
- Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update.Plants (Basel, Switzerland) · 2023Review
- Flavonoids - flowers, fruit, forage and the future.Journal of the Royal Society of New Zealand · 2023Review
- CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops.Molecular biotechnology · 2022Review
- Integrated network analyses identify MYB4R1 neofunctionalization in the UV-B adaptation of Tartary buckwheat.Plant communications · 2022Article
- Comparative Metabolome and Transcriptome Analysis of Anthocyanin Biosynthesis in White and Pink Petals of Cotton (International journal of molecular sciences · 2022Article
- Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World.Plants (Basel, Switzerland) · 2022Review
- Transcription Factors Evolve Faster Than Their Structural Gene Targets in the Flavonoid Pigment Pathway.Molecular biology and evolution · 2022Article
- ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3'-hydroxylase gene inFrontiers in plant science · 2022Article
- Integrative Analysis of Metabolome and Transcriptome Identifies Potential Genes Involved in the Flavonoid Biosynthesis inFrontiers in plant science · 2022Article
- The Flavonoid Biosynthesis Network in Plants.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Venation is a common anthocyanin pattern displayed in flowers that confers important ornamental traits to plants. An anthocyanin-related R2R3-MYB transcription factor, DPL, has been proposed to regulate corolla tube venation in petunia plants. Here, however, we provide evidence redefining the role of DPL in petunia. A CRISPR/Cas9-mediated mutation of DPL resulted in the absence of the vein-associated anthocyanin pattern above the abaxial surface of the flower bud, but not corolla tube venation, thus indicating that DPL did not regulate the formation of corolla tube venation. Alternately, quantitative real-time PCR analysis demonstrated that the spatiotemporal expression pattern of another R2R3-MYB gene, AN4, coincided with the formation of corolla tube venation in petunia. Furthermore, overexpression of AN4 promoted anthocyanin accumulation by increasing the expression of anthocyanin biosynthesis genes. CRISPR/Cas9-mediated mutation of AN4 led to an absence of corolla tube venation, suggesting that this gene in fact determines this key plant trait. Taken together, the results presented here redefine the prime regulator of corolla tube venation, paving the way for further studies on the molecular mechanisms underlying the various venation patterns in petunia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.