ArticleHorticulture research2021
SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Metabolism and regulation of chlorophyll and carotenoids in tomato fruits.Horticulture research · 2026Article
- The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network.BMC plant biology · 2026Review
- Review
- Characterization of a tomato chlh mis-sense mutant reveals a new function of ChlH in fruit ripening.Plant biotechnology journal · 2025Article
- Molecular breeding of tomato: Advances and challenges.Journal of integrative plant biology · 2025Review
- Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis.The Plant cell · 2024Review
- Integrative analysis of the methylome and transcriptome of tomato fruit (Horticulture research · 2024Article
- Genome-wide identification of GA2ox genes family and analysis of PbrGA2ox1-mediated enhanced chlorophyll accumulation by promoting chloroplast development in pear.BMC plant biology · 2024Article
- Guide RNA scaffold variants enabled easy cloning of large gRNA cluster for multiplexed gene editing.Plant biotechnology journal · 2024Article
- Article
- A Tomato EMS-Mutagenized Population Provides New Valuable Resources for Gene Discovery and Breeding of Developmental Traits.Plants (Basel, Switzerland) · 2022Article
- Post-translational regulation of metabolic checkpoints in plant tetrapyrrole biosynthesis.Journal of experimental botany · 2022Review
- Comparative Transcriptome Analysis Identified Key Pathways and Genes Regulating Differentiated Stigma Color in Melon (International journal of molecular sciences · 2022Article
- Genetic analysis of chlorophyll synthesis and degradation regulated by BALANCE of CHLOROPHYLL METABOLISM.Plant physiology · 2022Article
- Genetic Mapping and QTL Analysis of Stigma Color in Melon (Frontiers in plant science · 2022Article
- Regulation of tomato fruit elongation by transcription factor BZR1.7 through promotion ofHorticulture research · 2022Article
- Comparative transcriptomics analysis of developing peanut (Frontiers in plant science · 2022Article
- Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, chloroplasts are the sites at which photosynthesis occurs, and an increased abundance of chloroplasts increases the nutritional quality of plants and the resultant color of fruits. However, the molecular mechanisms underlying chlorophyll synthesis and chloroplast development in tomato fruits remain unknown. In this study, we isolated a chlorophyll-deficient mutant, reduced chlorophyll mutant 1 (rcm1), by ethylmethanesulfonate mutagenesis; this mutant produced yellowish fruits with altered chloroplast development. MutMap revealed that Solyc08g005010 is the causal gene underlying the rcm1 mutant phenotype. A single-nucleotide base substitution in the second exon of SlRCM1 results in premature termination of its translated protein. SlRCM1 encodes a chloroplast-targeted metalloendopeptidase that is orthologous to the BCM1 protein of Arabidopsis and the stay-green G protein of soybean (Glycine max L. Merr.). Notably, the yellowish phenotype of the lutescent1 mutant can be restored with the allele of SlRCM1 from wild-type tomato. In contrast, knockout of SlRCM1 by the CRISPR/Cas9 system in Alisa Craig yielded yellowish fruits at the mature green stage, as was the case for lutescent1. Amino acid sequence alignment and functional complementation assays showed that SlRCM1 is indeed Lutescent1. These findings provide new insights into the regulation of chloroplast development in tomato fruits.
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