Evidence map›Paper›PMID 34057719›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Multicolor Immunofluorescence Staining of Paraffin-Embedded Human Bone Marrow Sections.

Francesca M Panvini, Simone Pacini, Stefano Mazzoni, Simón Méndez-Ferrer

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
7.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Francesca M PanviniInstitute of Life Sciences, Sant'Anna School of Advanced Studies, Pisa, Italy.
Simone PaciniDepartment of Clinical and Experimental Medicine, Hematology Division, University of Pisa, Pisa, Italy.
Stefano MazzoniDepartment of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy.
Simón Méndez-FerrerWellcome-MRC Cambridge Stem Cell Institute, Cambridge, UK. sm2116@cam.ac.uk.
University of Pisa · ITNational Health Service · GBScuola Superiore Sant'Anna · IT

Funding

Cancer Research UK 26670Cancer Research UK C61367/A26670Medical Research Council MR/V005421/1Wellcome Trust 203151/Z/16/Z
6 · The paper itself

Abstract

Immunofluorescence is an indispensable method for the identification, localization and study of the expression of target antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections of human bone marrow. However, the procedure shows technical limitations because of the chemical and physical treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow trephine biopsies, improving the antigen-antibody recognition and preserving the morphology and the architecture of the bone marrow microenvironment.

Indexed as

Fluorescent Antibody TechniqueMicroscopy, FluorescenceParaffin EmbeddingAntigensBiomarkersBiopsyBone MarrowCellular MicroenvironmentColorHumansMicrowavesTissue FixationAntigensBiomarkersAntigen retrievalFFPEFixativeImmunofluorescenceMicrowave heating

Identifiers

PMID34057719
OpenAlexW3164086111

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.