ArticleNucleic acids research1988
Identification of regions essential for extrachromosomal replication and maintenance of an endogenous plasmid in Dictyostelium.
Article in Nucleic acids research, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Article
- Replication initiates at multiple dispersed sites in the ribosomal DNA plasmid of the protozoan parasite Entamoeba histolytica.Molecular and cellular biology · 1996Article
- The replication origin position and its relationship to a negative trans-acting transcription regulator encoded by Dictyostelium discoideum nuclear plasmid Ddp1.Current genetics · 1995Article
- Plasmid maintenance functions encoded on Dictyostelium discoideum nuclear plasmid Ddp1.Molecular and cellular biology · 1994Article
- Spatial and temporal expression of the Dictyostelium discoideum G alpha protein subunit G alpha 2: expression of a dominant negative protein inhibits proper prestalk to stalk differentiation.Molecular biology of the cell · 1994Article
- DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Amino acid substitutions in the Dictyostelium G alpha subunit G alpha 2 produce dominant negative phenotypes and inhibit the activation of adenylyl cyclase, guanylyl cyclase, and phospholipase C.Molecular biology of the cell · 1992Article
- A negative transcriptional control region of a developmentally-regulated gene co-localizes with the origin of replication of an endogenous plasmid in Dictyostelium.Nucleic acids research · 1992Article
- Expression of human antithrombin III in the cellular slime mould Dictyostelium discoideum.Applied microbiology and biotechnology · 1991Article
- Transcript and sequence analysis of a 5.1 kb contiguous fragment of Dictyostelium discoideum plasmid Ddp1 that contains the origin of replication and codes for several transcripts.Current genetics · 1990Article
- Molecular complementation of a genetic marker in Dictyostelium using a genomic DNA library.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Hygromycin resistance as a selectable marker in Dictyostelium discoideum.Molecular and cellular biology · 1989Article
- Sequence organization and gene expression of pGD1, a plasmid found in a wild isolate of Dictyostelium.Nucleic acids research · 1989Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initial experiments with the endogenous 12.3 kb Dictyostelium discoideum plasmid Ddp1 led to the generation of a large shuttle vector, Ddp1-20. In addition to Ddp1, this vector contains pBR322 and a gene fusion that confers G418 resistance in Dictyostelium cells. We have shown that Ddp1-20 replicates extrachromosomally in Dictyostelium cells and can be grown in Escherichia coli cells (1). We have now examined deletions within this vector to identify the elements essential for extrachromosomal replication and stable maintenance of the plasmid. We find that a 2.2 kb fragment is sufficient to confer stable, extrachromosomal replication with a reduction in copy number from about 40 to approximately 10-15 copies per cell. Vectors containing additional Ddp1 sequences have a higher copy number. The 2.2 kb region contains none of the complete, previously identified transcription units on Ddp1 expressed during vegetative growth or development. These results suggest that gene products expressed by Ddp1 are not essential for replication, stability, or partitioning of the plasmid between daughter cells. Vectors carrying only the 2.2 kb fragment plus the gene fusion conferring G418 resistance transform Dictyostelium cells with high efficiency using either calcium phosphate mediated transformation or electroporation. Finally, we have examined the relative levels of expression of actin promoters driving neoR genes when in extrachromosomal or integrating vectors.
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