ArticleACS omega2021
Surfactant-Free Decellularization of Porcine Aortic Tissue by Subcritical Dimethyl Ether.
Article in ACS omega, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Physical decellularization of fish skin utilizing electrical fields.Regenerative biomaterials · 2026Article
- Lipid Extraction from Wet Polymer-FlocculatedACS omega · 2025Article
- ECM-Preserving Decellularization of Dermis via Subtle Cell Membrane Disruption Induced by Subcritical Dimethyl Ether.ACS omega · 2025Article
- Extraction and Separation of Natural Products from Microalgae and Other Natural Sources Using Liquefied Dimethyl Ether, a Green Solvent: A Review.Foods (Basel, Switzerland) · 2024Review
- The journey of decellularized vessel: from laboratory to operating room.Frontiers in bioengineering and biotechnology · 2024Review
- Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase.Materials (Basel, Switzerland) · 2023Article
- Preliminary In Vitro Assessment of Decellularized Porcine Descending Aorta for Clinical Purposes.Journal of functional biomaterials · 2023Article
- Tensile Strength of Porcine Aorta Decellularized with Liquefied Dimethyl Ether and DNase.ACS omega · 2022Article
- Decellularized blood vessel development: Current state-of-the-art and future directions.Frontiers in bioengineering and biotechnology · 2022Review
- Preparation of Liposomes from Soy Lecithin Using Liquefied Dimethyl Ether.Foods (Basel, Switzerland) · 2021Article
- Enhancement of Lipid Extraction from Soya Bean by Addition of Dimethyl Ether as Entrainer into Supercritical Carbon Dioxide.Foods (Basel, Switzerland) · 2021Article
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine aortic tissue was decellularized by subcritical dimethyl ether (DME) used as an alternative to the surfactant sodium dodecyl sulfate. The process included three steps. For the first step, lipids were extracted from the porcine aorta using subcritical DME at 23 °C with a DME pressure of 0.56 MPa. Next, DME was evaporated from the aorta under atmospheric pressure and temperature. The second step involved DNA fragmentation by DNase, which was primarily identical to the common method. For the third step, similar to the common method, DNA fragments were removed by washing with water and ethanol. After 3 days of DNase treatment, the amount of DNA remaining in the porcine aorta was 40 ng/dry-mg, which was lower than the standard value of 50 ng/mg-dry. Hematoxylin and eosin staining showed that most cell nuclei were removed from the aorta. These results demonstrate that subcritical DME eliminates the need to utilize surfactants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.