ArticleIranian journal of biotechnology2020
Sequence and Phylogenetic Analysis of the First Complete Genome of Ricetungro spherical virus in Malaysia.
Article in Iranian journal of biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed, 4 citations in OpenAlex.
- Cymbidium Mosaic Virus Infecting Orchids: What, How, and What Next?Iranian journal of biotechnology · 2022Article
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8 authors at 1 institution in 1 country.
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Abstract
backgroundRice tungro disease (RTD) is a viral disease mainly affecting rice in Asia. RTD caused by
objectivesIn this study, we aimed to report the complete nucleotide sequence of Malaysian isolate of MATERIALS AND
methodsRTSV-SP isolate was isolated from a recent outbreak in a paddy field in Seberang Perai zone of Malaysia. Its complete genome was amplified by RT-PCR, cloned and sequenced.
resultsSequence analysis indicated that the genome of RTSV-SP consisted of 12,173 nucleotides (nt). Comparative analysis of 6 complete genome sequences using Clustal Omega showed that Seberang Perai isolate shared the highest nucleotide identity (96.04%) with Philippine-A isolate, except that the sORF-2 of RTSV-SP is shorter than RTSV Philippine-A by 27 amino acid residues. RTSV-SP found to cluster in Southeast Asia (SEA) group based on the whole genome sequence phylogenetic analysis using MEGA X software.
conclusionsPhylogenetic classification of RTSV isolates based on the complete nucleotide sequences showed more distinctive clustering pattern with the addition of RTSV-SP whole genome to the available isolates. Present study described the isolation and molecular characterization of RTSV-SP.
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