Evidence map›Paper›PMID 34056011›Full record

Observational studyJournal of immunology research2021

Effect of LPS on Cytokine Secretion from Peripheral Blood Monocytes in Juvenile Idiopathic Arthritis-Associated Uveitis Patients with Positive Antinuclear Antibody.

Jing Wang, Huiru Wu, Xinli Liu, Huiyu Jia, Hong Lu

Open access · goldAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Journal of immunology research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Jing WangDepartment of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.ORCID https://orcid.org/0000-0003-2794-1882
Huiru WuDepartment of Ophthalmology, The First Clinical Medicine College of Lanzhou University, Lanzhou 730000, China.ORCID https://orcid.org/0000-0001-6598-2568
Xinli LiuDepartment of Ophthalmology, Haidian Maternal and Child Health Hospital, Beijing 100080, China.ORCID https://orcid.org/0000-0002-4136-2062
Huiyu JiaDepartment of Ophthalmology, The First Clinical Medicine College of Lanzhou University, Lanzhou 730000, China.ORCID https://orcid.org/0000-0002-1045-5733
Hong LuDepartment of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.ORCID https://orcid.org/0000-0003-3873-9276
Capital Medical University · CNLanzhou University · CNHainan Maternal and Child Health Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesAntinuclear antibody (ANA) positivity is a key finding in JIA-associated uveitis (JIAU), but there are quite a few patients with negative ANA. There is no relevant report on the difference of their clinical manifestations. Previous animal model studies have found that the occurrence of uveitis is related to macrophage activation. In this article, our goal is to investigate changes in the morphology and cytokines of peripheral blood mononuclear cells (PBMCs) in uveitis patients testing positive or negative for ANAs after lipopolysaccharide (LPS) stimulation.

methodsA total of 30 patients were included in this study (10 in each group). They were divided into three groups (the ANA-positive [ANA+] group, ANA-negative [ANA-] group, and control group). There were ten patients (6 females and 4 males) in each group. Peripheral venous blood was collected into a heparinized tube, and PBMCs were isolated as soon as possible by the Ficoll-Hypaque density gradient separation method. Isolated cells were mixed with RPMI-1640 medium, and the cell concentration was adjusted to ensure that each patient had the same number of cells entering the study. After putting the extracted PBMC into the culture plate, LPS was added carefully to the plate. The cell culture supernatants were collected at 0 h, 3 h, 6 h, 12 h, and 24 h after LPS stimulation to detect the concentrations of IL-6, IL-1, TNF-

resultsThe newly isolated cells were approximately round. 6 h after LPS stimulation, the ratio of noncircular cells/circular cells was the highest in the ANA+ group. Unlike IL-10 that has been rising during the observation period, IL-6, IL-1, and TNF-

conclusionWith LPS motivation, cytokines in the ANA+ group increased the most violently.

Indexed as

AdolescentAntibodies, AntinuclearArthritis, JuvenileCells, CulturedChildChild, PreschoolCytokinesFemaleHumansLeukocytes, MononuclearLipopolysaccharidesMalePrimary Cell CultureUveitisAntibodies, AntinuclearCytokinesLipopolysaccharides

Identifiers

PMID34056011
PMCPMC8112907
OpenAlexW3158461529

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.