Evidence map›Paper›PMID 34055786›Full record

ArticleFrontiers in cell and developmental biology2021

A Putative Lipid-Associating Motif in the West Nile Virus NS4A Protein Is Required for Efficient Virus Replication.

Andrea Mikulasova, Leah K Gillespie, Rebecca L Ambrose, Turgut E Aktepe, Alice M Trenerry, Susann Liebscher, Jason M Mackenzie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Functional Roles and Host Interactions ofPathogens (Basel, Switzerland) · 2025
    Review
  2. West Nile virus unmasked: from gene variability to future challenges.Frontiers in cellular and infection microbiology · 2025
    Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Andrea MikulasovaDepartment of Physiology Anatomy and Microbiology, La Trobe University, Melbourne, VIC, Australia.
Leah K GillespieDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, Australia.
Rebecca L AmbroseDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, Australia.
Turgut E AktepeDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, Australia.
Alice M TrenerryDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, Australia.
Susann LiebscherDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, Australia.
Jason M MackenzieDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, Australia.
University of Melbourne · AULa Trobe University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Flavivirus replication is intimately associated with re-organized cellular membranes. These virus-induced changes in membrane architecture form three distinct membranous "organelles" that have specific functions during the flavivirus life cycle. One of these structures is the replication complex in which the flaviviral RNA is replicated to produce progeny genomes. We have previously observed that this process is strictly dependent on cellular cholesterol. In this study we have identified a putative cholesterol recognition/interaction amino acid consensus (CRAC) motif within the West Nile virus strain Kunjin virus (WNV

Indexed as

membrane remodelingNS4A proteinRNA replicationvirus-host interactionsWest Nile virus

Identifiers

PMID34055786
PMCPMC8149610
OpenAlexW3162661160

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.