Evidence map›Paper›PMID 34055784›Full record

ArticleFrontiers in cell and developmental biology2021

Single Cell Transcriptomic Analyses Reveal the Impact of bHLH Factors on Human Retinal Organoid Development.

Xiangmei Zhang, Igor Mandric, Kevin H Nguyen, Thao T T Nguyen, Matteo Pellegrini, James C R Grove, Steven Barnes, Xian-Jie Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Xiangmei ZhangDepartment of Ophthalmology, Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA, United States.
Igor MandricDepartment of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, United States.
Kevin H NguyenDepartment of Ophthalmology, Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA, United States.
Thao T T NguyenDepartment of Ophthalmology, Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA, United States.
Matteo PellegriniDepartment of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, United States.
James C R GroveDepartment of Ophthalmology, Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA, United States.
Steven BarnesDepartment of Ophthalmology, Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA, United States.
Xian-Jie YangDepartment of Ophthalmology, Stein Eye Institute, University of California, Los Angeles, Los Angeles, CA, United States.
University of California, Los Angeles · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
Neuroprotection Mechanism for PhotoreceptorsR01EY026319 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YANG, XIAN-JIE · 2016 to 2025
$4.1M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY026319
6 · The paper itself

Abstract

The developing retina expresses multiple bHLH transcription factors. Their precise functions and interactions in uncommitted retinal progenitors remain to be fully elucidated. Here, we investigate the roles of bHLH factors ATOH7 and Neurog2 in human ES cell-derived retinal organoids. Single cell transcriptome analyses identify three states of proliferating retinal progenitors: pre-neurogenic, neurogenic, and cell cycle-exiting progenitors. Each shows different expression profile of bHLH factors. The cell cycle-exiting progenitors feed into a postmitotic heterozygous neuroblast pool that gives rise to early born neuronal lineages. Elevating

Indexed as

bHLH factorshuman ES cellsneuronal differentiationretinal ganglion cellsretinal organoidsingle cell RNA-sequencing

Identifiers

PMID34055784
PMCPMC8155690
OpenAlexW3162636641

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.