Evidence map›Paper›PMID 34055032›Full record

ArticleNano today2021

Lateral size of graphene oxide determines differential cellular uptake and cell death pathways in Kupffer cells, LSECs, and hepatocytes.

Jiulong Li, Xiang Wang, Kuo-Ching Mei, Chong Hyun Chang, Jinhong Jiang, Xiangsheng Liu, Qi Liu, Linda M Guiney, Mark C Hersam, Yu-Pei Liao and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nano today, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 83 citations in OpenAlex.

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  18. A Lipid Perspective on Regulated Pyroptosis.International journal of biological sciences · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Jiulong LiCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Xiang WangCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Kuo-Ching MeiCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Chong Hyun ChangCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Jinhong JiangCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Xiangsheng LiuCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Qi LiuCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Linda M GuineyDepartments of Materials Science and Engineering, Chemistry, and Medicine, Northwestern University, Evanston, IL 60208, USA.
Mark C HersamDepartments of Materials Science and Engineering, Chemistry, and Medicine, Northwestern University, Evanston, IL 60208, USA.
Yu-Pei LiaoCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Huan MengCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
Tian XiaCenter of Environmental Implications of Nanotechnology (UC CEIN), University of California, Los Angeles, CA 90095, USA.
California NanoSystems Institute · USNorthwestern University · US

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
Engineered Nanomaterial Synthesis, Characterization and Method Development Center for Nano-safety ResearchU24ES026946 · NIEHS · HARVARD SCHOOL OF PUBLIC HEALTH · PI DEMOKRITOU, PHILIP · 2016 to 2020
$3.8M
Toxicological Profiling of Engineered Nanomaterials (ENMs) in the MPS (RES)U01ES027237 · NIEHS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI NEL, ANDRE ELIAS, XIA, TIAN · 2016 to 2020
$2.2M
NIDDK NIH HHS P30 DK041301NIEHS NIH HHS U01 ES027237NIEHS NIH HHS U24 ES026946
6 · The paper itself

Abstract

As a representative two-dimensional (2D) nanomaterial, graphene oxide (GO) has shown high potential in many applications due to its large surface area, high flexibility, and excellent dispersibility in aqueous solutions. These properties make GO an ideal candidate for bio-imaging, drug delivery, and cancer therapy. When delivered to the body, GO has been shown to accumulate in the liver, the primary accumulation site of systemic delivery or secondary spread from other uptake sites, and induce liver toxicity. However, the contribution of the GO physicochemical properties and individual liver cell types to this toxicity is unclear due to property variations and diverse cell types in the liver. Herein, we compare the effects of GOs with small (GO-S) and large (GO-L) lateral sizes in three major cell types in liver, Kupffer cells (KCs), liver sinusoidal endothelial cells (LSECs), and hepatocytes. While GOs induced cytotoxicity in KCs, they induced significantly less toxicity in LSECs and hepatocytes. For KCs, we found that GOs were phagocytosed that triggered NADPH oxidase mediated plasma membrane lipid peroxidation, which leads to PLC activation, calcium flux, mitochondrial ROS generation, and NLRP3 inflammasome activation. The subsequent caspase-1 activation induced IL-1β production and GSDMD-mediated pyroptosis. These effects were lateral size-dependent with GO-L showing stronger effects than GO-S. Amongst the liver cell types, decreased cell association and the absence of lipid peroxidation resulted in low cytotoxicity in LSECs and hepatocytes. Using additional GO samples with different lateral sizes, surface functionalities, or thickness, we further confirmed the differential cytotoxic effects in liver cells and the major role of GO lateral size in KUP5 pyroptosis by correlation studies. These findings delineated the GO effects on cellular uptake and cell death pathways in liver cells, and provide valuable information to further evaluate GO effects on the liver for biomedical applications.

Indexed as

graphene oxideGSDMD-dependent pyroptosislipid peroxidationNADPH oxidasephagocytosisPhospholipase C (PLC)

Identifiers

PMID34055032
PMCPMC8153408
OpenAlexW3115905980

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.