ArticleFrontiers in physiology2021
Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.
Article in Frontiers in physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Transgenerational effects of in ovo stimulation with synbiotic and choline on gonadal tissue across three generations.Scientific reports · 2025Article
- Functional analysis of lncRNAs in lipid metabolism of fat and lean line broiler embryonic livers.Poultry science · 2025Article
- Inter- and Transgenerational Effects of In Ovo Stimulation with Bioactive Compounds on Cecal Tonsils and Cecal Mucosa Transcriptomes in a Chicken Model.International journal of molecular sciences · 2025Article
- Screening of reliable reference genes for the normalization of RT-qPCR in chicken liver tissues and LMH cells.Scientific reports · 2024Article
- Effect of High Energy Low Protein Diet on Lipid Metabolism and Inflammation in the Liver and Abdominal Adipose Tissue of Laying Hens.Animals : an open access journal from MDPI · 2024Article
- Assessing avian leukosis virus proviral load and lesion correlates in fowl glioma-inducing virus-infected Japanese bantam chickens.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Study on the muscle transcriptome of two diverse Indian backyard poultry breeds acclimatized to different agro-ecological conditions.Molecular biology reports · 2023Article
- Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks.Frontiers in veterinary science · 2023Article
- Investigation of chicken housekeeping genes using next-generation sequencing data.Frontiers in genetics · 2022Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes. Therefore, it is very important to select stable reference genes to obtain accurate quantitative results. Although application examples of reference genes in mammals have been reported, no studies have investigated the use of reference genes in studying the growth and development of adipose tissue and the proliferation and differentiation of preadipocytes in chickens. In this study, GeNorm, a reference gene stability statistical algorithm, was used to analyze the expression stability of 14 candidate reference genes in the abdominal adipose tissue of broilers at 1, 4, and 7 weeks of age, the proliferation and differentiation of primary preadipocytes, as well as directly isolated preadipocytes and mature adipocytes. The results showed that the expression of the TATA box binding protein (
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