ArticleFrontiers in neuroscience2021
Optical Spike Detection and Connectivity Analysis With a Far-Red Voltage-Sensitive Fluorophore Reveals Changes to Network Connectivity in Development and Disease.
Article in Frontiers in neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- AI-Resolved Protein Energy Landscapes, Electrodynamics, and Fluidic Microcircuits as a Unified Framework for Predicting Neurodegeneration.International journal of molecular sciences · 2026Review
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- Optical segmentation-based compressed readout of neuronal voltage dynamics.Nature communications · 2025Article
- Article
- Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging.Scientific reports · 2024Article
- Electrophysiological Mechanisms and Validation of Ferritin-Based Magnetogenetics for Remote Control of Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Robust self-supervised denoising of voltage imaging data using CellMincer.bioRxiv : the preprint server for biology · 2024Article
- A red-emitting carborhodamine for monitoring and measuring membrane potential.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.Frontiers in neuroscience · 2024Review
- A red-emitting carborhodamine for monitoring and measuring membrane potential.bioRxiv : the preprint server for biology · 2023Article
- Predictable Fluctuations in Excitatory Synaptic Strength Due to Natural Variation in Presynaptic Firing Rate.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Imaging Spontaneous Neuronal Activity with Voltage-Sensitive Dyes.Current protocols · 2021Article
- Voltage Imaging with a NIR-Absorbing Phosphine Oxide Rhodamine Voltage Reporter.Journal of the American Chemical Society · 2021Article
- All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors.Frontiers in cellular neuroscience · 2021Article
- Imaging Voltage in Complete Neuronal Networks Within Patterned Microislands Reveals Preferential Wiring of Excitatory Hippocampal Neurons.Frontiers in neuroscience · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The ability to optically record dynamics of neuronal membrane potential promises to revolutionize our understanding of neurobiology. In this study, we show that the far-red voltage sensitive fluorophore, Berkeley Red Sensor of Transmembrane potential-1, or BeRST 1, can be used to monitor neuronal membrane potential changes across dozens of neurons at a sampling rate of 500 Hz. Notably, voltage imaging with BeRST 1 can be implemented with affordable, commercially available illumination sources, optics, and detectors. BeRST 1 is well-tolerated in cultures of rat hippocampal neurons and provides exceptional optical recording fidelity, as judged by dual fluorescence imaging and patch-clamp electrophysiology. We developed a semi-automated spike-picking program to reduce user bias when calling action potentials and used this in conjunction with BeRST 1 to develop an optical spike and connectivity analysis (OSCA) for high-throughput dissection of neuronal activity dynamics. The high temporal resolution of BeRST 1 enables dissection of firing rate changes in response to acute, pharmacological interventions with commonly used inhibitors like gabazine and picrotoxin. Over longer periods of time, BeRST 1 also tracks chronic perturbations to neurons exposed to amyloid beta 1-42 (Aβ
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