ArticleMolecular ecology resources2021
Optimal sample size for calibrating DNA methylation age estimators.
Article in Molecular ecology resources, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Epigenetic Clocks Can Predict Age in the Largemouth Bass-Complex Despite Spatial and Sexual Variation.Evolutionary applications · 2026Article
- VN1K is a pangenome-informed multi-omics and phenomics resource for the Vietnamese population.Nature communications · 2026Article
- Epigenetic Aging in Brain Tissue of the Self-Fertilizing Vertebrate,Ecology and evolution · 2026Article
- Searching for shared epigenetic clocks: evaluating ultra-conserved markers in a de novo genome assembly of the albacore tuna.GeroScience · 2026Article
- Genome-Wide DNA Methylation Patterns Predict Age in the Zebra Shark (Stegostoma tigrinum) and Provide Insight Into the Evolution of Vertebrate Aging.Molecular ecology · 2026Article
- An Epigenetic Clock for Accurate Age Prediction in Atlantic Cod Populations for Improved Fisheries Management.Molecular ecology resources · 2026Article
- Epigenetic Age Estimation for Hawaiian False Killer Whales (Pseudorca crassidens) in the Absence of 'Known-Age' Individuals.Molecular ecology resources · 2026Article
- Review
- Investigating epigenetic biomarkers of age, sex, and disease in captive South African cheetahs (Acinonyx jubatus jubatus).PloS one · 2026Article
- Dental Ageing Offers New Insights Into the First Epigenetic Clock for Common Dolphins (Ecology and evolution · 2025Article
- Dental aging offers new insights to the first epigenetic clock for common dolphins (bioRxiv : the preprint server for biology · 2025Article
- Artificial intelligence for comprehensive DNA methylation analysis: overview, challenges, and future directions.Briefings in bioinformatics · 2025Review
- Estimating Polar Bear (Ecology and evolution · 2025Article
- A Novel Framework for the Design of Minimized Epigenetic Clocks Using the Analysis of DNA Methylation Heterogeneity.International journal of molecular sciences · 2025Article
- A Sex-Specific Minimal CpG-Based Model for Biological Aging UsingInternational journal of molecular sciences · 2025Article
- Environmental DNA Epigenetics Accurately Predicts the Age of Cultured Fish Larvae.Ecology and evolution · 2025Article
- Application of DNA Methylation-Based Age Estimation to Construct an Age Structure of Humpback Whales in a Newly Emerged Wintering Ground Around Hachijojima Island, Tokyo Metropolis, Japan.Ecology and evolution · 2025Article
- The chloroplast genome sequences of Ipomoea alba and I. obscura (Convolvulaceae): genome comparison and phylogenetic analysis.Scientific reports · 2024Article
- Age-associated DNA methylation changes inEpigenetics · 2023Article
- Using epigenetic clocks to investigate changes in the age structure of critically endangered Māui dolphins.Ecology and evolution · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Age is a fundamental parameter in wildlife management as it is used to determine the risk of extinction, manage invasive species, and regulate sustainable harvest. In a broad variety of vertebrates species, age can be determined by measuring DNA methylation. Animals with known ages are initially required during development, calibration, and validation of these epigenetic clocks. However, wild animals with known ages are frequently difficult to obtain. Here, we perform Monte-Carlo simulations to determine the optimal sample size required to create an accurate calibration model for age estimation by elastic net regression modelling of cytosine-phosphate-guanine methylation data. Our results suggest a minimum calibration population size of 70, but ideally 134 individuals or more for accurate and precise models. We also provide estimates to the extent a model can be extrapolated beyond a distribution of ages that was used during calibration. The findings can assist researchers to better design age estimation models and decide if their model is adequate for determining key population attributes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.