ArticleMolecular ecology resources2022
A targeted amplicon sequencing panel to simultaneously identify mosquito species and Plasmodium presence across the entire Anopheles genus.
Article in Molecular ecology resources, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Local implementation of scalable malaria molecular surveillance in a low-resource setting: a North-South collaborative model.Malaria journal · 2026Article
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping of Aedes aegypti.Parasites & vectors · 2026Article
- SIMPLseq: a high-sensitivity Plasmodium falciparum genotyping and PCR contamination tracking tool.Malaria journal · 2026Article
- Article
- The Portable Microhaplotype Object and Tools.bioRxiv : the preprint server for biology · 2025Article
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping ofbioRxiv : the preprint server for biology · 2025Article
- A Short-Read Amplicon Sequencing Protocol and Bioinformatic Pipeline for Ecological Surveillance of Dipteran Disease Vectors.Molecular ecology resources · 2025Article
- Profiling insecticide resistance phenotypes and genotypes in Aedes aegypti populations across four regions in Puerto Rico.Scientific reports · 2025Article
- Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors.International journal of molecular sciences · 2025Review
- Application of a targeted amplicon sequencing panel to screen for insecticide resistance mutations in Anopheles darlingi populations from Brazil.Scientific reports · 2025Article
- MultiplexPeerJ · 2025Article
- The role of vector population variation and climate in Zika virus transmission patterns in Africa: a modelling study.The Lancet. Planetary health · 2024Article
- Entomological survey of sibling species in the Anopheles funestus group in Tanzania confirms the role of Anopheles parensis as a secondary malaria vector.Parasites & vectors · 2024Article
- Host preference patterns in domestic and wild settings: Insights intoEvolutionary applications · 2024Article
- Pathogen prospecting of museums: Reconstructing malaria epidemiology.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Low coverage of species constrains the use of DNA barcoding to assess mosquito biodiversity.Scientific reports · 2024Article
- Improved species assignments across the entireFrontiers in genetics · 2024Article
- AnoPrimer: Primer Design in malaria vectors informed by range-wide genomic variation.Wellcome open research · 2024Article
- Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel.Scientific reports · 2023Article
- Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay.Scientific reports · 2023Article
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Authors and funding
12 authors.
Funding
Abstract
Anopheles is a diverse genus of mosquitoes comprising over 500 described species, including all known human malaria vectors. While a limited number of key vector species have been studied in detail, the goal of malaria elimination calls for surveillance of all potential vector species. Here, we develop a multilocus amplicon sequencing approach that targets 62 highly variable loci in the Anopheles genome and two conserved loci in the Plasmodium mitochondrion, simultaneously revealing both the mosquito species and whether that mosquito carries malaria parasites. We also develop a cheap, nondestructive, and high-throughput DNA extraction workflow that provides template DNA from single mosquitoes for the multiplex PCR, which means specimens producing unexpected results can be returned to for morphological examination. Over 1000 individual mosquitoes can be sequenced in a single MiSeq run, and we demonstrate the panel's power to assign species identity using sequencing data for 40 species from Africa, Southeast Asia, and South America. We also show that the approach can be used to resolve geographic population structure within An. gambiae and An. coluzzii populations, as the population structure determined based on these 62 loci from over 1000 mosquitoes closely mirrors that revealed through whole genome sequencing. The end-to-end approach is quick, inexpensive, robust, and accurate, which makes it a promising technique for very large-scale mosquito genetic surveillance and vector control.
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