Evidence map›Paper›PMID 34053021›Full record

ReviewAdvances in experimental medicine and biology2021

Super-resolution Microscopy for Biological Imaging.

Zhigang Yang, Soham Samanta, Wei Yan, Bin Yu, Junle Qu

Abstract readReview
PubMed Publisher
In one paragraph

Review in Advances in experimental medicine and biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Clustering within a single-component biomolecular condensate.bioRxiv : the preprint server for biology · 2025
    Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhigang YangCenter for Biomedical Phonics, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Soham SamantaCenter for Biomedical Phonics, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Wei YanCenter for Biomedical Phonics, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Bin YuCenter for Biomedical Phonics, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Junle QuCenter for Biomedical Phonics, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China. jlqu@szu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studying the ultra-fine structures and functions of the subcellular organelles and exploring the dynamic biological events in depth are the key issues in contemporary biological research. Fluorescence bio-imaging has been used to study cell biology for decades. However, the structures and functions of the subcellular organelles which fall under the diffraction limit are still not explored fully at a nanoscale level. Several super-resolution microscopy (SRM) techniques have been devised over the years which can be utilized to overcome diffraction limit. These techniques have opened a new window in biological research. However, SRM methods are highly vulnerable to the lack of appropriate fluorophores and other sophisticated technical considerations. Therefore, this chapter briefly summarizes the basic principles of various SRM methods which have been frequently utilized in biological imaging. The chapter not only gives an overview of the technical advantages and drawbacks about using different SRM techniques for bio-imaging applications but also briefly articulates the nitty-gritties of selecting a proper fluorescent probe for a specific SRM experiment with biological samples.

Indexed as

Fluorescent DyesOptical ImagingMicroscopy, FluorescenceOrganellesFluorescent DyesBiological imagingPhoto-Activated Localization Microscopy (PALM/FPALM)Stimulated Emission Depletion (STED) microscopyStochastic Optical Reconstruction Microscopy (STORM)Super-resolution microscopySuper-resolution Optical Fluctuation Imaging (SOFI)

Identifiers

PMID34053021

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.