ArticleJournal of assisted reproduction and genetics2021
Dysregulation of bisphosphoglycerate mutase during in vitro maturation of oocytes.
Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- GM-CSF increases glucose uptake and mitochondrial activity and reduces reactive oxygen species levels in mouse cumulus-oocyte complexes during oocyte in vitro maturation.The Journal of reproduction and development · 2026Article
- Single-cell transcriptome sequencing reveals the mechanism of Realgar improvement on erythropoiesis in mice with myelodysplastic syndrome.Cancer cell international · 2025Article
- Construction and validation of a machine learning model integrating ultrasound features and inflammatory markers (OVART-ML) for predicting ovarian torsion and ischemic necrosis risk in children.Frontiers in pediatrics · 2025Article
- Addition of granulocyte macrophage colony stimulating factor (GM-CSF) duringThe Journal of reproduction and development · 2024Article
- Addition of GM-CSF during in vitro oocyte maturation improves embryo development and implantation and birth rate in mice.Reproduction & fertility · 2024Article
- Cellular atlas of the human ovary using morphologically guided spatial transcriptomics and single-cell sequencing.Science advances · 2024Article
- Effect of In Vitro Maturation of Human Oocytes Obtained After Controlled Ovarian Hormonal Stimulation on the Expression of Development- and Zona Pellucida-Related Genes and Their Interactions.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Optical imaging detects metabolic signatures associated with oocyte quality†.Biology of reproduction · 2022Article
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Authors and funding
5 authors.
Funding
Abstract
purposeOxygen is vital for oocyte maturation; however, oxygen regulation within ovarian follicles is not fully understood. Hemoglobin is abundant within the in vivo matured oocyte, indicating potential function as an oxygen regulator. However, hemoglobin is significantly reduced following in vitro maturation (IVM). The molecule 2,3-bisphosphoglycerate (2,3-BPG) is essential in red blood cells, facilitating release of oxygen from hemoglobin. Towards understanding the role of 2,3-BPG in the oocyte, we characterized gene expression and protein abundance of bisphosphoglycerate mutase (Bpgm), which synthesizes 2,3-BPG, and whether this is altered under low oxygen or hemoglobin addition during IVM.
methodsHemoglobin and Bpgm expression within in vivo matured human cumulus cells and mouse cumulus-oocyte complexes (COCs) were evaluated to determine physiological levels of Bpgm. During IVM, Bpgm gene expression and protein abundance were analyzed in the presence or absence of low oxygen (2% and 5% oxygen) or exogenous hemoglobin.
resultsThe expression of Bpgm was significantly lower than hemoglobin when mouse COCs were matured in vivo. Following IVM at 20% oxygen, Bpgm gene expression and protein abundance were significantly higher compared to in vivo. At 2% oxygen, Bpgm was significantly higher compared to 20% oxygen, while exogenous hemoglobin resulted in significantly lower Bpgm in the COC.
conclusionHemoglobin and 2,3-BPG may play a role within the maturing COC. This study shows that IVM increases Bpgm within COCs compared to in vivo. Decreasing oxygen concentration and the addition of hemoglobin altered Bpgm, albeit not to levels observed in vivo.
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