Evidence map›Paper›PMID 34052872›Full record

ArticleArchives of microbiology2021

Chemical composition and potentiating action of Norfloxacin mediated by the essential oil of Piper caldense C.D.C. against Staphylococcus aureus strains overexpressing efflux pump genes.

Antonio Linkoln Alves Borges Leal, Camila Fonseca Bezerra, Camila Confortin, Luiz Everson da Silva, Emanuelle Machado Marinho, Márcia Machado Marinho, Mayron Alves Vasconcelos, Terezinha Gonçalves da Silva, Emmanuel Silva Marinho, Alexandre Magno Rodrigues Teixeira and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Antibacterial Activity of the Essential Oil ofPlants (Basel, Switzerland) · 2023
    Article
  4. Review
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 1 country.

Antonio Linkoln Alves Borges LealDepartment of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil.
Camila Fonseca BezerraDepartment of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, PE, Brazil.
Camila ConfortinPostgraduate Programme in Sustainable Territorial, Federal University of Paraná, Curitiba, PR, Brazil.
Luiz Everson da SilvaPostgraduate Programme in Sustainable Territorial, Federal University of Paraná, Curitiba, PR, Brazil.
Emanuelle Machado MarinhoDepartment of Analytical Chemistry and Physical Chemistry, Federal University of Ceará, Fortaleza, CE, Brazil.
Márcia Machado MarinhoFaculty of Education, Science and Letters of Iguatu, State University of Ceará, Iguatu, CE, Brazil.
Mayron Alves VasconcelosMinas Gerais State University, Divinópolis Unit, MG, Brazil.
Terezinha Gonçalves da SilvaDepartment of Antibiotics, Drug Planning and Synthesis Laboratory-LPSF, Federal University of Pernambuco, Cidade Universitária, Recife, PE, Brazil.
Emmanuel Silva MarinhoGroup of Theoretical Chemistry and Electrochemistry, State University of Ceará, Campus FAFIDAM, Limoeiro do Norte, Fortaleza, CE, Brazil.
Alexandre Magno Rodrigues TeixeiraDepartment of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil.
Henrique Douglas Melo CoutinhoDepartment of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil.
Humberto Medeiros BarretoDepartment of Microbiology and Parasitolog, Federal University of Piaui, Teresina, PI, Brazil.
Hélcio Silva Dos SantosDepartment of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil. helciodossantos@gmail.com.ORCID http://orcid.org/0000-0001-5527-164X
Universidade Estadual do Ceará · BRUniversidade Federal de Pernambuco · BRUniversidade Federal do Paraná · BRUniversidade Federal do Piauí · BRUniversidade Regional do Cariri · BRState University of Vale do Acaraú · BRUniversidade do Estado de Minas Gerais · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Infectious diseases caused by multidrug-resistant microorganisms has increased in the last years. Piper species have been reported as a natural source of phytochemicals that can help in combating fungal and bacterial infections. This study had as objectives characterize the chemical composition of the essential oil from Piper caldense (EOPC), evaluate its potential antimicrobial activity, and investigate the synergistic effect with Norfloxacin against multidrug-resistant S. aureus overproducing efflux pumps, as well as, verify the EOPC ability to inhibit the Candida albicans filamentation. EOPC was extracted by hydrodistillation, and the chemical constituents were identified by gas chromatography, allowing the identification of 24 compounds (91.9%) classified as hydrocarbon sesquiterpenes (49.6%) and oxygenated sesquiterpenes (39.5%). Antimicrobial tests were performed using a 96-well plate microdilution method against C. albicans ATCC 10231, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 standard strains, as well as against multidrug-resistant strains S. aureus SA1199B (overexpressing norA gene), S. aureus K2068 (overexpressing mepA gene) and S. aureus K4100 (overexpressing qacC gene). The oil showed activity against C. albicans ATCC 10231 (≥ 512 µg/mL) and was able to inhibit hyphae formation, an important mechanism of virulence of C. albicans. On the other hand, EOPC was inactive against all bacterial strains tested (≤ 1,024 µg mL). However, when combined with Norfloxacin at subinhibitory concentration EOPC reduced the Norfloxacin and Ethidium bromide MIC values against S. aureus strains SA1199B, K2068 and K4100. These results indicate that EOPC is a source of phytochemicals acting as NorA, MepA and QacC inhibitors.

Indexed as

Bacterial ProteinsMethicillin-Resistant Staphylococcus aureusNorfloxacinOils, VolatilePiperStaphylococcus aureusAnti-Bacterial AgentsATP-Binding Cassette, Sub-Family C ProteinsMicrobial Sensitivity TestsAnti-Bacterial AgentsATP-Binding Cassette, Sub-Family C ProteinsBacterial ProteinsNorfloxacinOils, VolatileAntibacterialAntifungalEfflux pump inhibitorsEssential oilFungal dimorphismPiper caldense

Identifiers

PMID34052872
PMCPMC8164493
OpenAlexW3164579904

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.