Evidence map›Paper›PMID 34050480›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors.

Sandrine Picaud, Bertrand Lambolez, Ludovic Tricoire

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sandrine PicaudNeuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS), Sorbonne Université UM119, CNRS UMR8246, INSERM U1130, Paris, France.
Bertrand LambolezNeuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS), Sorbonne Université UM119, CNRS UMR8246, INSERM U1130, Paris, France. bertrand.lambolez@upmc.fr.
Ludovic TricoireNeuroscience Paris Seine-Institut de Biologie Paris Seine (NPS-IBPS), Sorbonne Université UM119, CNRS UMR8246, INSERM U1130, Paris, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optogenetic calcium sensors enable the imaging in real-time of the activities of single or multiple neurons in brain slices and in vivo. Bioluminescent probes engineered from the natural calcium sensor aequorin do not require illumination, are virtually devoid of background signal, and exhibit wide dynamic range and low cytotoxicity. These probes are thus well suited for long-duration, whole-field recordings of multiple neurons simultaneously. Here, we describe a protocol for monitoring and analyzing the dynamics of neuronal ensembles using whole-field bioluminescence imaging of an aequorin-based sensor in brain slice.

Indexed as

AequorinAnimalsBiosensing TechniquesBrainCalciumFluorescence Resonance Energy TransferLuminescent AgentsLuminescent MeasurementsMiceNeural PathwaysNeuronsOptical ImagingAequorinCalciumLuminescent AgentsBioluminescenceCalcium imagingCoelenterazine (CTZ)Genetically encoded probeGFP-aequorinNeuronNeuronal ensembleViral transfer

Identifiers

PMID34050480

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.