Evidence map›Paper›PMID 34050469›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe.

Tadanobu Takahashi, Yuuki Kurebayashi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, Takashi Suzuki

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Tadanobu TakahashiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan. takahasi@u-shizuoka-ken.ac.jp.
Yuuki KurebayashiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
Tadamune OtsuboDepartment of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International University, Hiroshima, Japan.
Kiyoshi IkedaDepartment of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International University, Hiroshima, Japan.
Akira MinamiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
Takashi SuzukiDepartment of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
University of Shizuoka · JPHiroshima International University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Visualization of virus-infected cells is usually performed by immunostaining with an antiviral antibody. On the other hand, we established an easy method for fluorescence (FL) imaging of cells infected with influenza A and B viruses and some paramyxoviruses without the need for cell fixation and an antiviral antibody. These viruses and the cells they have infected express the viral surface enzyme "neuraminidase" or "hemagglutinin-neuraminidase" that shows sialidase activity. Sialidase activity is fluorescently visualized by using a sialidase fluorogenic probe developed in our previous study. The probe enables histochemical FL imaging of the virus-infected cells and is applicable to virus isolation and detection of an influenza virus resistant to antiinfluenza drugs of sialidase inhibitors.

Indexed as

Drug Resistance, ViralFluorescenceAnimalsAntiviral AgentsChlorocebus aethiopsCOS CellsDogsMadin Darby Canine Kidney CellsNeuraminidaseOptical ImagingOrthomyxoviridaeOrthomyxoviridae InfectionsVero CellsAntiviral AgentsNeuraminidaseDrug resistanceFluorescence imagingImaging probeInfected cellsInfluenza virusNeuraminidaseParamyxovirusSialidaseVirus isolation

Identifiers

PMID34050469
OpenAlexW3164941794

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.