ArticleJournal of assisted reproduction and genetics2021
A threshold concentration of FSH is needed during IVM of ex vivo collected human oocytes.
Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05370794 (Is in Vitro Maturation of Oocytes Influenced by the Origin of Gonadotropins Used in Maturation Medium), which is not on this map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Is in Vitro Maturation of Oocytes Influenced by the Origin of Gonadotropins Used in Maturation Medium: is There a Difference in Efficiency When Using Urinary Versus Recombinant Produced Follicle Stimulating Hormone and Human Chorionic Gonadotropin
Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Transcriptional Changes in Cumulus Cells During In Vitro Maturation of Human Oocytes.Molecular reproduction and development · 2025Article
- Regulating human oocyte maturation in vitro: a hypothesis based on oocytes retrieved from small antral follicles during ovarian tissue cryopreservation.Journal of assisted reproduction and genetics · 2025Article
- Meeting proceedings: International Society for Fertility Preservation Tokyo, 15-17 November, 2024.Journal of assisted reproduction and genetics · 2025Article
- Maturational competence of equine oocytes is associated with alterations in their 'cumulome'.Molecular human reproduction · 2024Article
- Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes.Journal of assisted reproduction and genetics · 2024Article
- In vitro matured oocytes have a higher developmental potential than in vivo matured oocytes after hormonal ovarian stimulation in Callithrix jacchus.Journal of ovarian research · 2024Article
- Moving the context of preserving fertility into an expanded domain.Journal of assisted reproduction and genetics · 2023Article
- IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture.International journal of molecular sciences · 2023Article
- Oocyte diameter predicts the maturation rate of human immature oocytes collected ex vivo.Journal of assisted reproduction and genetics · 2022Article
- Concentrations of oocyte secreted GDF9 and BMP15 decrease with MII transition during human IVM.Reproductive biology and endocrinology : RB&E · 2022Article
- Validating Reference Gene Expression Stability in Human Ovarian Follicles, Oocytes, Cumulus Cells, Ovarian Medulla, and Ovarian Cortex Tissue.International journal of molecular sciences · 2022Article
- Clinical Outcomes ofFrontiers in endocrinology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the effect of different FSH concentrations on human oocyte maturation in vitro and its impact on gene expression of key factors in the surrounding cumulus cells.
methodsThe study included 32 patients who underwent unilateral oophorectomy for ovarian tissue cryopreservation (OTC) (aged 28 years on average). Immature oocytes were collected from surplus medulla tissue. A total of 587 immature oocytes were divided into three categories according to the size of the cumulus mass: large (L-COCs), small (S-COCs), and naked oocytes (NOs), and submitted to 44-h IVM with one of the following concentrations of recombinant FSH: 0 IU/L, 20 IU/L, 40 IU/L, 70 IU/L, or 250 IU/L. After IVM, oocyte nuclear maturation stage and diameter were recorded. The relative gene expression of FSHR, LHCGR, and CYP19A1 in cumulus cells before (day 0; D0) and after IVM were evaluated.
resultsAddition of 70 or 250 IU/L FSH to the IVM medium improved oocyte nuclear maturation compared to 0, 20, and 40 IU/L FSH by upregulating LHCGR and downregulating FSHR in the cumulus cells.
conclusionFSH improved oocyte nuclear maturation at concentrations above 70 IU/L suggesting a threshold for FSH during IVM of ex vivo collected human oocytes from small antral follicles. Moreover, current results for the first time highlight that FSH function in vitro is mediated via cumulus cells by downregulating FSHR and upregulating LHCGR, which was also observed when the immature oocytes progressed in meiosis from the GV to the MII stage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.