Evidence map›Paper›PMID 34049541›Full record

ArticleMicrobial cell factories2021

Genome-based selection and application of food-grade microbes for chickpea milk fermentation towards increased L-lysine content, elimination of indigestible sugars, and improved flavour.

Muzi Tangyu, Michel Fritz, Rosa Aragao-Börner, Lijuan Ye, Biljana Bogicevic, Christoph J Bolten, Christoph Wittmann

Open access · goldAbstract read
In one paragraph

Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 29 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Muzi TangyuInstitute of Systems Biotechnology, Saarland University, Saarbrücken, Germany.
Michel FritzInstitute of Systems Biotechnology, Saarland University, Saarbrücken, Germany.
Rosa Aragao-BörnerNestlé Research Center, Lausanne, Switzerland.
Lijuan YeNestlé Research Center, Lausanne, Switzerland.
Biljana BogicevicNestlé Research Center, Lausanne, Switzerland.
Christoph J BoltenNestlé Product Technology Center Food, Singen, Germany.
Christoph WittmannInstitute of Systems Biotechnology, Saarland University, Saarbrücken, Germany. christoph.wittmann@uni-saarland.de.ORCID http://orcid.org/0000-0002-7952-985X
Nestlé (Switzerland) · CHSaarland University · DENestlé (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPlant-based milk alternatives are more popular than ever, and chickpea-based milks are among the most commercially relevant products. Unfortunately, limited nutritional value because of low levels of the essential amino acid L-lysine, low digestibility and unpleasant taste are challenges that must be addressed to improve product quality and meet consumer expectations.

resultsUsing in-silico screening and food safety classifications, 31 strains were selected as potential L-lysine producers from approximately 2,500 potential candidates. Beneficially, 30% of the isolates significantly accumulated amino acids (up to 1.4 mM) during chickpea milk fermentation, increasing the natural level by up to 43%. The best-performing strains, B. amyloliquefaciens NCC 156 and L. paracasei subsp. paracasei NCC 2511, were tested further. De novo lysine biosynthesis was demonstrated in both strains by

conclusionsB. amyloliquefaciens NCC 156 and L. paracasei subsp. paracasei NCC 2511 emerged as multi-benefit microbes for chickpea milk fermentation with strong potential for industrial processing of the plant material. Given the high number of L-lysine-producing isolates identified in silico, this concept appears promising to support strain selection for food fermentation.

Indexed as

Biosynthetic PathwaysCicerFermentationFlavoring AgentsFood MicrobiologyGenome, BacterialLactobacillalesLysineMilk SubstitutesSugarsTasteFlavoring AgentsLysineSugars13C isotope studyBacillus amyloliquefaciensChickpeafermentationFlavourIndigestible sugarLactic acid bacteriaLacticaseibacillus paracaseiL-lysinePlant-based milk alternativePlant milkRaffinoseStachyose

Identifiers

PMID34049541
PMCPMC8161961
OpenAlexW3164481401

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.