ArticleBMC musculoskeletal disorders2021
Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.
Article in BMC musculoskeletal disorders, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- A spatiotemporal atlas of senescence-associated secretory phenotype in fracture healing: stage-dependent mechanisms and therapeutic windows.Inflammation and regeneration · 2026Review
- Monocyte cluster identification during the early stage of fracture healing in an ovariectomized mouse model.Frontiers in medicine · 2026Article
- Neuroimmune regulation of post-traumatic bone regeneration: focus on inflammatory switching and functional recovery.Frontiers in immunology · 2026Review
- Bone and Cardiometabolic Connection: Crosstalk between Osteoporosis and Atherosclerosis.Cell biochemistry and biophysics · 2025Review
- Temporal Molecular Signatures of Early Human Clavicle Fracture Healing: Characterization of Hematological, Cytokine, and miRNA Profiles.International journal of molecular sciences · 2025Article
- Bone Mineral Density and Intermuscular Fat Derived from Computed Tomography Images Using Artificial Intelligence Are Associated with Fracture Healing.Bioengineering (Basel, Switzerland) · 2025Article
- Article
- Macrophages in osteoporotic fractures: from immunometabolic mechanisms to precision therapeutic approaches.Frontiers in endocrinology · 2025Review
- Fortified Withaferin A accelerates the transition from fibrovascular to bone remodeling phase during endochondral bone formation to promote ossification.Frontiers in endocrinology · 2025Article
- Inhibition of inflammatory osteoclasts accelerates callus remodeling in osteoporotic fractures by enhancing CGRPCell death and differentiation · 2024Article
- Article
- Temporal dynamics of immune-stromal cell interactions in fracture healing.Frontiers in immunology · 2024Review
- Monotropein Protects Mesenchymal Stem Cells from Lipopolysaccharide-Induced Impairments and Promotes Fracture Healing in an Ovariectomized Mouse Model.Calcified tissue international · 2023Article
- Regulon active landscape reveals cell development and functional state changes of human primary osteoblasts in vivo.Human genomics · 2023Article
- The role of macrophages in fracture healing: a narrative review of the recent updates and therapeutic perspectives.Stem cell investigation · 2023Review
- Fracture Healing in the Setting of Endocrine Diseases, Aging, and Cellular Senescence.Endocrine reviews · 2022Review
- Article
- Sesamin Promotes Osteoporotic Fracture Healing by Activating Chondrogenesis and Angiogenesis Pathways.Nutrients · 2022Article
- Association of IL-6 and TGF-β Gene Polymorphisms with the Risk of Thoracolumbar Osteoporotic Vertebral Compression Fractures.Pharmacogenomics and personalized medicine · 2022Article
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMacrophages and inflammatory cytokines play important roles in bone fracture healing. However, the expression patterns of macrophages and inflammatory cytokines during fracture healing under the condition of postmenopausal osteoporosis have not been fully revealed.
methodsTibia transverse fracture was established 12 weeks after ovariectomy or sham operation in 16-week old female mice. Tibias were harvested before fracture or 1, 3, 5, 7, 14, 21, 28 days after fracture for radiological and histological examinations. M1/M2 inflammatory macrophages, osteal macrophages and gene expressions of tumor necrosis factor-α, interleukin-6, interleukin-1β and macrophage conversion related molecules in the fracture haematoma or callus were also detected.
resultsThe processes of fracture healing, especially the phases of endochondral ossification and callus remodeling, were delayed in ovariectomized mice. The expressions of tumor necrosis factor-α and interleukin-6, but not interleukin-1β, in the fracture haematoma or callus were disturbed. Expressions of tumor necrosis factor-α were decreased at 1, 14 and 21 days post-fracture (DPF), and were increased at 3, 5 and 7 DPF. Interleukin-6 expressions at 1, 3 and 21 DPF were significantly increased. We found the decreases in M1 and M2 macrophages at 1 DPF of the initial inflammatory stage. M2 macrophages at 14 DPF of the middle stage and osteal macrophages at 14, 21 and 28 DPF of the middle and late stages of fracture healing were also reduced in ovariectomized mice.
conclusionsThe expressions of macrophages and inflammatory cytokines were impaired in ovariectomized mice, which might contribute partially to poor fracture healing.
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