ArticleMethods in molecular biology (Clifton, N.J.)2021
Cecal Slurry Injection in Neonatal and Adult Mice.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Metabolic-epigenetic rewiring of CCR5Science advances · 2026Article
- Comparison of Laparotomy and Percutaneous Intraperitoneal Lavage on Survival in a Cecal Slurry Peritonitis Model in Preterm Mice.The Kobe journal of medical sciences · 2026Article
- HIF-1α Promotes Macrophage Extracellular Trap Formation and Exacerbates Acute Lung Injury in Neonatal Sepsis.Biomedicines · 2026Article
- Optimization of Preclinical Rodent Research Models of Human Shock: Part One Intra-Abdominal Sepsis.Shock (Augusta, Ga.) · 2026Review
- Sepsis modelling: current approaches and organ-on-chip perspectives.EBioMedicine · 2026Review
- HLA DP/DRA molecule regulates systemic inflammation and neuroinflammation, aggravates cognitive impairment and long-term anxiety in murine model of sepsis-associated encephalopathy.Frontiers in immunology · 2026Article
- An antibody targeting high-molecular-weight kininogen blocks contact system activation in a model of polymicrobial sepsis.Journal of thrombosis and haemostasis : JTH · 2025Article
- Article
- Modeling pathogen-driven neonatal late-onset sepsis: a modification to the murine cecal slurry.Frontiers in cellular and infection microbiology · 2025Article
- Human colon stem cells are the predominant epithelial responders to bacterial antigens.Frontiers in immunology · 2025Article
- Claudin-2 upregulation enhances intestinal permeability, immune activation, dysbiosis, and mortality in sepsis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- M1 cholinergic signaling in the brain modulates cytokine levels and splenic cell sub-phenotypes following cecal ligation and puncture.Molecular medicine (Cambridge, Mass.) · 2024Article
- An IL-10/DEL-1 axis supports granulopoiesis and survival from sepsis in early life.Nature communications · 2024Article
- Experimental Sepsis Models: Advantages and Limitations.The Eurasian journal of medicine · 2023Article
- The progress to establish optimal animal models for the study of acute-on-chronic liver failure.Frontiers in medicine · 2023Review
- Severe gut mucosal injury induces profound systemic inflammation and spleen-associated lymphoid organ response.Frontiers in immunology · 2023Article
- Anti-Inflammatory and Antibacterial Effects and Mode of Action of Greek Arbutus, Chestnut, and Fir Honey in Mouse Models of Inflammation and Sepsis.Microorganisms · 2022Article
- A Toolbox to Investigate the Impact of Impaired Oxygen Delivery in Experimental Disease Models.Frontiers in medicine · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Studying the pathophysiology of sepsis still requires animal models, and the mouse remains the most commonly used species. Here we discuss the "cecal slurry" (CS) model of polymicrobial, peritoneal sepsis and compare and contrast it to other commonly used methods. Among the different murine models of sepsis, cecal ligation and puncture (CLP), and not the CS, is often considered the "gold standard" to induce polymicrobial sepsis in laboratory animals. CLP is a well-described model involving a simple surgical procedure that closely mimics the clinical course of intra-abdominal sepsis. However, CLP may not be an option for experiments involving newborn pups, where the cecum is indistinguishable from small bowel, where differences in microbiome content may affect the experiment, or where surgical procedures/anesthesia exposure needs to be limited. An important alternative method is the CS model, involving the intraperitoneal injection of cecal contents from a donor animal into the peritoneal cavity of a recipient animal to induce polymicrobial sepsis. Furthermore, CS is an effective alternative model of intraperitoneal polymicrobial sepsis in adult mice and can now be considered the "gold standard" for experiments in neonatal mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.