ArticleMethods in molecular biology (Clifton, N.J.)2021
In Situ Localization of Plant Lipid Metabolites by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI).
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Lipid MRI in plant science: principles and potential areas of application.Journal of experimental botany · 2026Review
- Lipid regulation inFrontiers in plant science · 2026Review
- Bridging the gaps: advanced techniques to unlock lipid function in plant reproductive development.Plant reproduction · 2025Review
- Ferroptosis and Metabolic Dysregulation: Emerging Chemical Targets in Cancer and Infection.Molecules (Basel, Switzerland) · 2025Review
- Flexible substrate-based mass spectrometry platform for in situ non-destructive molecular imaging of living plants.Plant biotechnology journal · 2025Article
- Imaging plant metabolism in situ.Journal of experimental botany · 2024Review
- Applications of mass spectrometry imaging in botanical research.Advanced biotechnology · 2024Review
- Effective Mechanisms for Improving Seed Oil Production in Pennycress (Frontiers in plant science · 2022Article
- Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues.Frontiers in chemistry · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a major analytical platform for the determination and localization of lipid metabolites directly from tissue sections. Unlike analysis of lipid extracts, where lipid localizations are lost due to homogenization and/ or solvent extraction, MALDI-MSI analysis is capable of revealing spatial localization of metabolites while simultaneously collecting high chemical resolution mass spectra. Important considerations for obtaining high quality MALDI-MS images include tissue preservation, section preparation, MS data collection and data processing. Errors in any of these steps can lead to poor quality metabolite images and increases the chance for metabolite misidentification and/ or incorrect localization. Here, we present detailed methods and recommendations for specimen preparation, MALDI-MS instrument parameters, software analysis platforms for data processing, and practical considerations for each of these steps to ensure acquisition of high-quality chemical and spatial resolution data for reconstructing MALDI-MS images of plant tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.