Evidence map›Paper›PMID 34047990›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC).

Ze-Hua Guo, Mee-Len Chye

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Ze-Hua GuoSchool of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
Mee-Len ChyeSchool of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China. mlchye@hku.hk.
Chinese University of Hong Kong · CNUniversity of Hong Kong · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isothermal titration calorimetry (ITC) is a quantitative, biophysical method to investigate intermolecular binding between biomolecules by directly measuring the heat exchange in the binding reaction. The assay is carried out in solution when the molecules interact in vitro. This allows to determine values for binding affinity (K

Indexed as

Acyl Coenzyme ACalorimetryCarrier ProteinsChromatography, LiquidDiazepam Binding InhibitorEntropyHot TemperatureKineticsLigandsLipidsOryzaProtein BindingProteinsThermodynamicsAcyl Coenzyme ACarrier ProteinsDiazepam Binding InhibitorLigandsLipidsProteinsAcyl-CoA thioestersHis-tagged proteinsLiquid chromatographyOryza sativaProtein–ligand interactionProtein–protein interactionProtein purification

Identifiers

PMID34047990
OpenAlexW3164871728

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.