Evidence map›Paper›PMID 34047986›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Collection and Analysis of Phloem Lipids.

Susanne Hoffmann-Benning

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Phloem Sap Composition: What Have We Learnt from Metabolomics?International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Susanne Hoffmann-BenningDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA. hoffma16@msu.edu.
Michigan State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The plant phloem is a long-distance conduit for the transport of assimilates but also of mobile developmental and stress signals. These signals can be sugars, metabolites, amino acids, peptides, proteins, microRNA, or mRNA. Yet small lipophilic molecules such as oxylipins and, more recently, phospholipids have emerged as possible long-distance signals as well. Analysis of phloem (phospho)lipids, however, requires enrichment, purification, and sensitive analysis. This chapter describes the EDTA-facilitated approach of phloem exudate collection, phase partitioning against chloroform-methanol for lipid separation and enrichment, and analysis/identification of phloem lipids using LC-MS with multiplexed collision induced dissociation (CID).

Indexed as

ArabidopsisArabidopsis ProteinsChromatography, Thin LayerLipid MetabolismLipidsMembrane LipidsPhloemPlant CellsPlantsRNA, MessengerSugarsTandem Mass SpectrometryArabidopsis ProteinsLipidsMembrane LipidsRNA, MessengerSugarsLipidsLiquid chromatography–mass spectrometry (LC-MS)Long-distance lipid-mediated signalingMultiplexed collision induced dissociation (CID)Phloem

Identifiers

PMID34047986
OpenAlexW3164814961

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.