ArticleMethods in molecular biology (Clifton, N.J.)2021
Analysis of Free and Esterified Sterol Content and Composition in Seeds Using GC and ESI-MS/MS.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Insecticidal and Repellent Activity of Different Pomegranate Peel Extracts Against Granary Weevil Adults.Insects · 2025Article
- Phytosterol organic acid esters: Characterization, anti-inflammatory properties and a delivery strategy to improve mitochondrial function.Current research in food science · 2024Article
- Advances and Challenges in Plant Sterol Research: Fundamentals, Analysis, Applications and Production.Molecules (Basel, Switzerland) · 2023Review
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
Total sterol content and composition in plant tissues can be easily determined by gas chromatography (GC) after saponification of the total lipid extract. However, in oleogenic tissues a significant proportion of the sterol is esterified to fatty acids, with GC methodologies unable to provide information about the proportion and the molecular species composition of intact steryl esters (SEs). Here we describe an electrospray ionization-tandem mass spectrometry (ESI-MS/MS) and Multiple Reaction Monitoring (MRM) method which, in parallel with GC analysis, allows for the accurate determination of both free and esterified sterol content and composition in seeds. After extraction of seed oil with hexane, free sterols are derivatized with undecanoyl chloride, total steryl esters are then purified from triacylglycerol (TAG) by liquid chromatography, infused and ionized as ammonium adducts, with molecular species identified and quantified by fragmentation in the presence of internal standards.
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