ArticleMethods in molecular biology (Clifton, N.J.)2021
Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Review
- Complex sphingolipid metabolism impacts cell division and plasmodesmal development in the moss Physcomitrium patens.Plant physiology · 2025Article
- Automated mass spectrometry-based profiling of multi-glycosylated glycosyl inositol phospho ceramides (GIPC) reveals specific series GIPC rearrangements during barley grain development and heat stress response.The Plant journal : for cell and molecular biology · 2025Article
- Identification of INOSITOL PHOSPHORYLCERAMIDE SYNTHASE 2 (IPCS2) as a new rate-limiting component in Arabidopsis pathogen entry control.The Plant journal : for cell and molecular biology · 2025Article
- Plasticity of the Arabidopsis leaf lipidome and proteome in response to pathogen infection and heat stress.Plant physiology · 2025Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Exploring the plant lipidome: techniques, challenges, and prospects.Advanced biotechnology · 2024Review
- Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC-ESI-QqTOF with parallel reaction monitoring.Scientific reports · 2023Article
- Heat stress leads to rapid lipid remodeling and transcriptional adaptations in Nicotiana tabacum pollen tubes.Plant physiology · 2022Article
- Plastidic membrane lipids are oxidized by a lipoxygenase inFrontiers in plant science · 2022Article
- HILIC-ESI-MS analysis of phosphatidic acid methyl esters artificially generated during lipid extraction from microgreen crops.Journal of mass spectrometry : JMS · 2021Article
- Sphingolipid Δ4-desaturation is an important metabolic step for glycosylceramide formation in Physcomitrium patens.Journal of experimental botany · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant lipidome is highly complex and changes dynamically under the influence of various biotic and abiotic stresses. Targeted analyses based on mass spectrometry enable the detection and characterization of the plant lipidome. It can be analyzed in plant tissues of different developmental stages and from isolated cellular organelles and membranes. Here, we describe a sensitive method to establish the relative abundance of molecular lipid species belonging to three lipid categories: glycerolipids, sphingolipids, and sterol lipids. The method is based on a monophasic lipid extraction and includes the derivatization of a few rare and low-abundant lipid classes. The molecular lipid species are resolved by lipid class-specific reverse-phase liquid chromatography and detected by nanoelectrospray ionization coupled with tandem mass spectrometry. The triple quadrupole analyzer is used for detection with multiple reaction monitoring (MRM). Mass transition lists are constructed based on the knowledge of organism-specific lipid building blocks. They are initially determined by classical lipid analytical methods and then used for combinative assembly of all possible lipid structures. The targeted analysis enables detailed and comprehensive profiling of the entire lipid content and composition of plants.
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