Evidence map›Paper›PMID 34046698›Full record

ArticleAnalytical and bioanalytical chemistry2021

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples.

Nadja E Solis-Marcano, Myreisa Morales-Cruz, Gabriela Vega-Hernández, Ramón Gómez-Moreno, Claudia Binder, Abel Baerga-Ortiz, Craig Priest, Carlos R Cabrera

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Nadja E Solis-MarcanoDepartment of Chemistry, Molecular Sciences Research Center, University of Puerto Rico, Río Piedras Campus, San Juan, 00925-2537, Puerto Rico.
Myreisa Morales-CruzDepartment of Chemistry, Molecular Sciences Research Center, University of Puerto Rico, Río Piedras Campus, San Juan, 00925-2537, Puerto Rico.
Gabriela Vega-HernándezDepartment of Chemistry, Molecular Sciences Research Center, University of Puerto Rico, Río Piedras Campus, San Juan, 00925-2537, Puerto Rico.
Ramón Gómez-MorenoDepartment of Biochemistry, Molecular Sciences Research Center, University of Puerto Rico, Medical Sciences Campus, San Juan, 00936-5067, Puerto Rico.
Claudia BinderFuture Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, Adelaide, South Australia, 5095, Australia.
Abel Baerga-OrtizDepartment of Biochemistry, Molecular Sciences Research Center, University of Puerto Rico, Medical Sciences Campus, San Juan, 00936-5067, Puerto Rico.
Craig PriestFuture Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, Adelaide, South Australia, 5095, Australia.
Carlos R CabreraDepartment of Chemistry, Molecular Sciences Research Center, University of Puerto Rico, Río Piedras Campus, San Juan, 00925-2537, Puerto Rico. carlos.cabrera2@upr.edu.ORCID http://orcid.org/0000-0002-3342-8666
University of Puerto Rico at Río Piedras · PRUniversity of Puerto Rico, Medical Sciences Campus · PRUniversity of South Australia · AU

Funding

Upgrade Laboratories Facilities at UPR-Rio PiedrasR25GM061151 · NIGMS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI CARBALLEIRA, NESTOR MANUEL · 2001 to 2023
$32.5M
NASA NNX10AM80HNIGMS NIH HHS R25 GM061151NIH-RISE 5R25GM061151NSF-CREST 1736093
6 · The paper itself

Abstract

A fast PCR-assisted impedimetric biosensor was developed for the selective detection of the clbN gene from the polyketide synthase (pks) genomic island in real Escherichia coli samples. This genomic island is responsible for the production of colibactin, a harmful genotoxin that has been associated with colorectal cancer. The experimental protocol consisted of immobilizing the designated forward primer onto an Au electrode surface to create the sensing probe, followed by PCR temperature cycling in blank, positive, and negative DNA controls. Target DNA identification was possible by monitoring changes in the system's charge transfer resistance values (R

Indexed as

Genome, BacterialBiosensing TechniquesDielectric SpectroscopyEscherichia coliLimit of DetectionPeptidesPolyketidesPolyketide SynthasesPolymerase Chain ReactioncolibactinPeptidesPolyketidesPolyketide SynthasesDNA biosensorElectrochemical impedance spectroscopyPolyketide synthase genomic islandPolymerase chain reaction (PCR)

Identifiers

PMID34046698
PMCPMC8159250
OpenAlexW3164345978

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.