ReviewCellular and molecular life sciences : CMLS2021
Recent progress in mass spectrometry-based strategies for elucidating protein-protein interactions.
Review in Cellular and molecular life sciences : CMLS, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- The multidimensional significance of virus-host protein interactions and their implications in the antiviral defense of plants.Stress biology · 2026Review
- Strategies for constructing context-specific protein-protein interaction networks.Briefings in bioinformatics · 2026Review
- Label-Free Target Discovery Strategy for Natural Active Products.Biomolecules · 2026Review
- Proteomic Technology.Advances in experimental medicine and biology · 2026Review
- gPPIpred: A User-Friendly PPI Predictor Based on Protein Molecular Graphs.microPublication biology · 2026Article
- Thianthrenium Chemistry for Identification of Protein-Protein Interactions in Cells.Journal of the American Chemical Society · 2025Article
- Decoding protein dynamics with limited proteolysis coupled to mass spectrometry: A comprehensive review.Journal of pharmaceutical analysis · 2025Review
- Metabolic control of glycosylation forms for establishing glycan-dependent protein interaction networks.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Developing a new cleavable crosslinker reagent for in-cell crosslinking.Communications chemistry · 2025Article
- In vivo regulation of an endogenously tagged protein by a light-regulated kinase.G3 (Bethesda, Md.) · 2025Article
- TMEM138: From Biological Functions to Diseases.Physiological research · 2025Review
- Simplicity within biological complexity.Bioinformatics advances · 2025Article
- Graph-Theoretical Prediction and Analysis of Biologically Relevant Substructures in an Open and Closed Conformation of Respiratory Complex I.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Immunoaffinity Depletion of High-Abundance Proteins from Serum/Plasma for Proteomic Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Improved protein interaction models predict differences in complexes between human cell lines.bioRxiv : the preprint server for biology · 2024Article
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.ACS measurement science au · 2024Review
- Review
- INTREPPPID-an orthologue-informed quintuplet network for cross-species prediction of protein-protein interaction.Briefings in bioinformatics · 2024Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
Protein-protein interactions are fundamental to various aspects of cell biology with many protein complexes participating in numerous fundamental biological processes such as transcription, translation and cell cycle. MS-based proteomics techniques are routinely applied for characterising the interactome, such as affinity purification coupled to mass spectrometry that has been used to selectively enrich and identify interacting partners of a bait protein. In recent years, many orthogonal MS-based techniques and approaches have surfaced including proximity-dependent labelling of neighbouring proteins, chemical cross-linking of two interacting proteins, as well as inferring PPIs from the co-behaviour of proteins such as the co-fractionating profiles and the thermal solubility profiles of proteins. This review discusses the underlying principles, advantages, limitations and experimental considerations of these emerging techniques. In addition, a brief account on how MS-based techniques are used to investigate the structural and functional properties of protein complexes, including their topology, stoichiometry, copy number and dynamics, are discussed.
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Registered trials
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