Evidence map›Paper›PMID 34045785›Full record

ArticleProceedings of SPIE--the International Society for Optical Engineering2019

Hyperspectral imaging fluorescence excitation scanning (HIFEX) microscopy for live cell imaging.

Silas J Leavesley, John Robert Griswold, Joshua Deal, Kathleen McAlister, Sam Mayes, Craig Browning, Marina Parker, Samantha Gunn Mayes, Thomas C Rich

Abstract read
In one paragraph

Article in Proceedings of SPIE--the International Society for Optical Engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Silas J LeavesleyDepartment of Chemical and Biomolecular Engineering, University of South Alabama.
John Robert GriswoldDepartment of Chemical and Biomolecular Engineering, University of South Alabama.
Joshua DealDepartment of Pharmacology, University of South Alabama.
Kathleen McAlisterDepartment of Biomedical Sciences, University of South Alabama.
Sam MayesDepartment of Chemical and Biomolecular Engineering, University of South Alabama.
Craig BrowningDepartment of Chemical and Biomolecular Engineering, University of South Alabama.
Marina ParkerDepartment of Chemical and Biomolecular Engineering, University of South Alabama.
Samantha Gunn MayesDepartment of Chemical and Biomolecular Engineering, University of South Alabama.
Thomas C RichDepartment of Pharmacology, University of South Alabama.

Funding

National Dissemination of I-Corps@NCATS: Accelerating Translation through CommercializationUL1TR003096 · NCATS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI GUTIERREZ, ORLANDO M, KIMBERLY, ROBERT P. · 2019 to 2023
$43.6M
T-type Calcium Channels and von Willebrand Factor ReleaseP01HL066299 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI LEAVESLEY, SILAS JOSIAH · 2001 to 2022
$34.6M
UAB Center for Clinical and Translational Science (CCTS)UL1TR001417 · NCATS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI KIMBERLY, ROBERT P. · 2015 to 2018
$31.4M
Functional Diversity of Compartmentalized Calcium Signaling in Airway Smooth MuscleR01HL137030 · NHLBI · THOMAS JEFFERSON UNIVERSITY · PI DESHPANDE, DEEPAK A · 2018 to 2021
$2.2M
Request for a Spectral Confocal MicroscopeS10RR027535 · NCRR · UNIVERSITY OF SOUTH ALABAMA · PI RICH, THOMAS C · 2010 to 2010
$397k
Andor Revolution WD Confocal Imaging SystemS10OD020149 · OD · UNIVERSITY OF SOUTH ALABAMA · PI TAYLOR, MARK STEPHEN · 2015 to 2015
$395k
NCATS NIH HHS UL1 TR001417NCATS NIH HHS UL1 TR003096NCRR NIH HHS S10 RR027535NHLBI NIH HHS P01 HL066299NHLBI NIH HHS R01 HL137030NIH HHS S10 OD020149
6 · The paper itself

Abstract

In the past two decades, spectral imaging technologies have expanded the capacity of fluorescence microscopy for accurate detection of multiple labels, separation of labels from cellular and tissue autofluorescence, and analysis of autofluorescence signatures. These technologies have been implemented using a range of optical techniques, such as tunable filters, diffraction gratings, prisms, interferometry, and custom Bayer filters. Each of these techniques has associated strengths and weaknesses with regard to spectral resolution, spatial resolution, temporal resolution, and signal-to-noise characteristics. We have previously shown that spectral scanning of the fluorescence excitation spectrum can provide greatly increased signal strength compared to traditional emission-scanning approaches. Here, we present results from utilizing a Hyperspectral Imaging Fluorescence Excitation Scanning (HIFEX) microscope system for live cell imaging. Live cell signaling studies were performed using HEK 293 and rat pulmonary microvascular endothelial cells (PMVECs), transfected with either a cAMP FRET reporter or a Ca

Indexed as

BiopsyClassificationEEMFingerprintOpticalSignatureSpectralSpectroscopy

Identifiers

PMID34045785
PMCPMC8151178

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.