ArticleScientific reports2021
Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Multi-omics and low-input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos.FEBS open bio · 2026Article
- Variable partition of non-homogeneous ooplasm sets the stage for divergent potency of 2-cell stage blastomeres.Molecular human reproduction · 2026Article
- Impact of TGF-β and ERK1/2 inhibition on preimplantation embryo development and quality.Iranian journal of basic medical sciences · 2026Article
- Nuclear Dynamics and Its Timing Regulation Revealed by Live-Cell Imaging.Advances in experimental medicine and biology · 2026Review
- Totipotency or plenipotency: rethinking stem cell bipotentiality.Current opinion in genetics & development · 2025Review
- Proteogenomic reprogramming to a functional human blastomere-like stem cell state via a PARP-DUX4 regulatory axis.Cell reports · 2025Article
- Male gamete copies to characterize genome inheritance and generate progenies.Scientific reports · 2025Article
- Animal and vegetal materials of mouse oocytes segregate at first zygotic cleavage: a simple mechanism that makes the two-cell blastomeres differ reciprocally from the start.Molecular human reproduction · 2025Article
- Current Status of Synthetic Mammalian Embryo Models.International journal of molecular sciences · 2024Review
- Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoids.Developmental cell · 2024Article
- The Dynamics of Histone Modifications during Mammalian Zygotic Genome Activation.International journal of molecular sciences · 2024Review
- A comprehensive review: synergizing stem cell and embryonic development knowledge in mouse and human integrated stem cell-based embryo models.Frontiers in cell and developmental biology · 2024Review
- Navigating stem cell culture: insights, techniques, challenges, and prospects.Frontiers in cell and developmental biology · 2024Review
- Omics Views of Mechanisms for Cell Fate Determination in Early Mammalian Development.Genomics, proteomics & bioinformatics · 2023Review
- In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans.International journal of molecular sciences · 2023Review
- Reduction, proliferation, and differentiation defects of stem cells over time: a consequence of selective accumulation of old centrioles in the stem cells?Molecular biology reports · 2023Review
- Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures.Frontiers in cell and developmental biology · 2023Article
- Cloning by SCNT: Integrating Technical and Biology-Driven Advances.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Lab partners: oocytes, embryos and company. A personal view on aspects of oocyte maturation and the development of monozygotic twins.Animal reproduction · 2023Article
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Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In multicellular organisms, oocytes and sperm undergo fusion during fertilization and the resulting zygote gives rise to a new individual. The ability of zygotes to produce a fully formed individual from a single cell when placed in a supportive environment is known as totipotency. Given that totipotent cells are the source of all multicellular organisms, a better understanding of totipotency may have a wide-ranging impact on biology. The precise delineation of totipotent cells in mammals has remained elusive, however, although zygotes and single blastomeres of embryos at the two-cell stage have been thought to be the only totipotent cells in mice. We now show that a single blastomere of two- or four-cell mouse embryos can give rise to a fertile adult when placed in a uterus, even though blastomere isolation disturbs the transcriptome of derived embryos. Single blastomeres isolated from embryos at the eight-cell or morula stages and cultured in vitro manifested pronounced defects in the formation of epiblast and primitive endoderm by the inner cell mass and in the development of blastocysts, respectively. Our results thus indicate that totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.