Evidence map›Paper›PMID 34045521›Full record

ArticleScientific reports2021

Visualisation and biovolume quantification in the characterisation of biofilm formation in Mycoplasma fermentans.

Ammar A Awadh, Alison F Kelly, Gary Forster-Wilkins, David Wertheim, Richard Giddens, Simon W Gould, Mark D Fielder

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Microorganisms · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. A Comprehensive Survey with Quantitative Comparison of Image Analysis Methods for Microorganism Biovolume Measurements.Archives of computational methods in engineering : state of the art reviews · 2023
    Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ammar A AwadhSchool of Life Science Pharmacy, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK.
Alison F KellySchool of Life Science Pharmacy, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK.
Gary Forster-WilkinsSchool of Life Science Pharmacy, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK.
David WertheimSchool of Computer Science and Mathematics, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK.
Richard GiddensSchool of Life Science Pharmacy, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK.
Simon W GouldSchool of Life Science Pharmacy, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK.
Mark D FielderSchool of Life Science Pharmacy, Chemistry Faculty of Science, Engineering and Computing, Kingston University London, Kingston, UK. m.fielder@kingston.ac.uk.
Kingston University · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability of mycoplasmas to persist on surfaces has been widely acknowledged, despite their fastidious nature. However, the organism's capability to form a recognisable biofilm structure has been identified more recently. In the current study Mycoplasma fermentans was found to adhere to the glass surface forming highly differentiated biofilm structures. The volumes of biofilm microcolonies were quantified and observed to be greater at late growth stage than those at early growth stage. The channel diameters within biofilms were measured with Scanning Electron Microscopy images and found to be consistent with the size observed in Confocal Laser Scanning Microscope images. The combination of imaging methods with 3D visualisation provides key findings that aid understanding of the mycoplasma biofilm formation and true biofilm architecture. The observations reported here provide better understanding of the persistence of these minimalist pathogens in nature and clinical settings.

Indexed as

Mycoplasma fermentansBiofilmsMicroscopy, Confocal

Identifiers

PMID34045521
PMCPMC8160185
OpenAlexW3164709314

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.