ArticleJournal of translational medicine2021
The accumulation of exosome-associated microRNA-1246 and microRNA-150-3p in human red blood cell suspensions.
Article in Journal of translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 19 citations in OpenAlex.
- Y-RBCEVs accelerate fracture repair through osteogenesis and VCAN-mediated CD44/PI3K/AKT-dependent macrophage polarization.Bioactive materials · 2027Article
- Molecular Mechanisms of Transfusion-Associated Immunomodulation and Its Impact in the Critically Ill.International journal of molecular sciences · 2025Review
- Erythrocyte Extracellular Vesicles Amalgamate into the Hair and Skin to Maintain Homeostasis.Journal of extracellular vesicles · 2025Article
- Human erythrocytes' perplexing behaviour: erythrocytic microRNAs.Molecular and cellular biochemistry · 2025Review
- Measurement of α-synuclein as protein cargo in plasma extracellular vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- MiR-33a-5p in stored red blood cells regulates genes of innate immune response and promotes inflammation.Aging · 2024Article
- Apoptotic vesicles derived from human red blood cells promote bone regeneration via carbonic anhydrase 1.Cell proliferation · 2024Article
- Whispers in the Blood: Leveraging MicroRNAs for Unveiling Autologous Blood Doping in Athletes.International journal of molecular sciences · 2023Review
- MicroRNAs as Quality Assessment Tool in Stored Packed Red Blood Cell in Blood Banks.Journal of blood medicine · 2023Review
- Exploration of Exosomal miRNAs from Serum and Synovial Fluid in Arthritis Patients.Diagnostics (Basel, Switzerland) · 2022Article
- Circulating exo-miR-154-5p regulates vascular dementia through endothelial progenitor cell-mediated angiogenesis.Frontiers in cellular neuroscience · 2022Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransfusion-related immunomodulation (TRIM) can be caused by exosomes, in which case, microRNAs (miRNAs) are one critical factor impacting exosome behavior. This study aims to investigate and analyze the expression profiles of exosomal miRNA in red blood cell (RBC) suspensions during storage and to identify potential TRIM-related miRNAs as well as their potential functions.
methodsA total of 25 packs of RBC suspensions were randomly collected. Exosome were extracted by ultracentrifugation and then identified and characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and western blot (WB). Exosomal miRNA profiles were acquired using gene chips in five packs on week 1 and week 5. The expression data were compared from the two time points identifying accumulated miRNAs with statistical significance and their predicted targeting genes were analyzed. Based on the gene chip results, quantitative reverse transcription-polymerase chain reactions (qRT-PCR) were performed to verify miRNA accumulation in the rest 20 packs sampling on week 1, 3 and 5.
resultsGene chip analysis revealed that most exosomal miRNAs were enriched as the storage period progressed. Compared to samples from week 1, week 5 samples exhibited a total of 539 differential miRNA expressions, among which, 159 were statistically significant (P < 0.05) and 148 (93.08%) were accumulated. In the bioinformatics functional analysis, significant immunoregulatory annotations related to the thyroid hormone, mitogen-activated protein kinase (MAPK), focal adhesion and RAS signaling pathways were identified. The top 17 differential expression miRNAs were validated by qRT-PCR. The results confirmed that all the 17 miRNAs were accumulated with increasing storage time. In particular, miRNA-1246 and miRNA-150-3p were the most enriched strands by more than 150-folds in the 5-week storage period.
conclusionsAs storage progressed, numerous exosomal miRNAs accumulated in the RBC suspensions, which are informatically connected to multiple immuno-signaling pathways. MiRNA-1246 and miRNA-150-3p may be essential mediators impacting the immunoregulation functions of exosomes in RBC suspensions, considering their significant accumulating scales. Further research should therefore focus on the relationship between these miRNAs and TRIM.
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