ArticlePLoS pathogens2021
The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli.
Article in PLoS pathogens, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Small Regulatory RNAs in Prokaryotes: Key Features, Identification, Environmental Roles, and Applications.Microorganisms · 2026Review
- Orchestrating infection: the impact of RyfA and TimR sRNAs on stress resistance and virulence in avian pathogenicApplied and environmental microbiology · 2026Article
- Host range and zoonotic potential linked to P-like fimbrial (PLF) adhesin specificity in avian pathogenic Escherichia coli.PLoS pathogens · 2026Article
- The Landscape and Regulatory Determinants of A-to-I RNA Editing in Escherichia coli and Pseudomonas aeruginosa Isolated From Patients With Urinary Tract and Ear Infections.The Journal of infectious diseases · 2026Article
- A small RNA fromVirulence · 2025Article
- Tiny but Mighty: Small RNAs-The Micromanagers of Bacterial Survival, Virulence, and Host-Pathogen Interactions.Non-coding RNA · 2025Review
- The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response.BMC microbiology · 2024Article
- Type I toxin-antitoxin systems in bacteria: from regulation to biological functions.EcoSal Plus · 2024Review
- ProQ binding to small RNA RyfA promotes virulence and biofilm formation in avian pathogenic Escherichia coli.Veterinary research · 2023Article
- Urinary Tract Infections Caused by UropathogenicInternational journal of molecular sciences · 2023Review
- The Klebsiella pneumoniaeInfection and immunity · 2023Article
- Article
- Simulated Colonic Fluid Replicates theInfection and immunity · 2022Article
- Article
- Key players in regulatory RNA realm of bacteria.Biochemistry and biophysics reports · 2022Review
- Involvement ofInternational journal of molecular sciences · 2022Article
- Three Microbial Musketeers of the Seas:International journal of molecular sciences · 2022Article
- Do small RNAs unlock the below ground microbiome-plant interaction mystery?Frontiers in molecular biosciences · 2022Review
- What Flips the Switch? Signals and Stress Regulating Extraintestinal PathogenicMicroorganisms · 2021Review
- Flagella, Type I Fimbriae and Curli of UropathogenicMicroorganisms · 2021Article
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Authors and funding
11 authors.
Funding
Abstract
Urinary tract infections (UTIs) are a common bacterial infectious disease in humans, and strains of uropathogenic Escherichia coli (UPEC) are the most frequent cause of UTIs. During infection, UPEC must cope with a variety of stressful conditions in the urinary tract. Here, we demonstrate that the small RNA (sRNA) RyfA of UPEC strains is required for resistance to oxidative and osmotic stresses. Transcriptomic analysis of the ryfA mutant showed changes in expression of genes associated with general stress responses, metabolism, biofilm formation and genes coding for cell surface proteins. Inactivation of ryfA in UPEC strain CFT073 decreased urinary tract colonization in mice and the ryfA mutant also had reduced production of type 1 and P fimbriae (pili), adhesins which are known to be important for UTI. Furthermore, loss of ryfA also reduced UPEC survival in human macrophages. Thus, ryfA plays a key regulatory role in UPEC adaptation to stress, which contributes to UTI and survival in macrophages.
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