ArticlePloS one2021
Binding and neutralization of C. difficile toxins A and B by purified clinoptilolite-tuff.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Safety and efficacy of purified clinoptilolite-tuff treatment in patients with irritable bowel syndrome with diarrhea: Randomized controlled trial.World journal of gastroenterology · 2022Trial
- Topically administered purified clinoptilolite-tuff for the treatment of cutaneous wounds: A prospective, randomised phase I clinical trial.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2022Trial
- High Sorption Efficiency of Purified Clinoptilolite-Tuff for Aflatoxins B1 and M1: A Case Study in Plant-Based Beverages and Milk.International journal of molecular sciences · 2025Article
- Review of Applications of Zeolites in Dermatology: Molecular Perspectives and Translational Potentials.International journal of molecular sciences · 2025Review
- Caffeic acid phenethyl ester protectseLife · 2025Article
- Unveiling the Broad-Spectrum Virucidal Potential of Purified Clinoptilolite-Tuff.Microorganisms · 2024Article
- Purified Clinoptilolite-Tuff as an Efficient Sorbent for Food-Derived Peanut Allergens.International journal of molecular sciences · 2024Article
- Antimicrobial Guanidinylate Polycarbonates Show Oral In Vivo Efficacy Against Clostridioides Difficile.Advanced healthcare materials · 2024Article
- Binding and inactivation of human coronaviruses, including SARS-CoV-2, onto purified clinoptilolite-tuff.Scientific reports · 2023Article
- Purified Clinoptilolite-Tuff as an Efficient Sorbent for Gluten Derived from Food.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridioides difficile (C. difficile) infection is a major public health problem worldwide. The current treatment of C. difficile-associated diarrhea relies on the use of antibacterial agents. However, recurrences are frequent. The main virulence factors of C. difficile are two secreted cytotoxic proteins toxin A and toxin B. Alternative research exploring toxin binding by resins found a reduced rate of recurrence by administration of tolevamer. Hence, binding of exotoxins may be useful in preventing a relapse provided that the adsorbent is innocuous. Here, we examined the toxin binding capacity of G-PUR®, a purified version of natural clinoptilolite-tuff. Our observations showed that the purified clinoptilolite-tuff adsorbed clinically relevant amounts of C. difficile toxins A and B in vitro and neutralized their action in a Caco-2 intestinal model. This conclusion is based on four independent sets of findings: G-PUR® abrogated toxin-induced (i) RAC1 glucosylation, (ii) redistribution of occludin, (iii) rarefaction of the brush border as visualized by scanning electron microscopy and (iv) breakdown of the epithelial barrier recorded by transepithelial electrical resistance monitoring. Finally, we confirmed that the epithelial monolayer tolerated G-PUR® over a wide range of particle densities. Our findings justify the further exploration of purified clinoptilolite-tuff as a safe agent in the treatment and/or prevention of C. difficile-associated diarrhea.
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