Evidence map›Paper›PMID 34043183›Full record

ArticleEndocrine2021

Quantitative proteomics revealed the molecular characteristics of distinct types of granulated somatotroph adenomas.

Yifan Tang, Tao Xie, Silin Wu, Qiaoqiao Yang, Tengfei Liu, Chen Li, Shuang Liu, Zhiyong Shao, Xiaobiao Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Endocrine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yifan Tang *Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Tao Xie *Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Silin Wu *Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Qiaoqiao YangInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
Tengfei LiuDepartment of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Chen LiDepartment of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Shuang LiuDepartment of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Zhiyong ShaoDepartment of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Xiaobiao ZhangDepartment of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China. xiaobiao_zhang@163.com.ORCID 0000-0003-3970-1106

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeSomatotroph adenomas are obviously heterogeneous in clinical characteristics, imaging performance, pathological diagnosis and therapeutic effect. The heterogeneity of the tumors, especially for SG and DG type adenomas, have attracted great interest in identifying the specific pathological markers and therapeutic targets of them. However, previous analyses of the molecular characteristics of the subtypes of somatotroph adenomas were performed at genomic and transcriptome level. The proteomic differences between the two subtypes of somatotroph adenomas are still unknown.

methodsTumor samples were surgically removed from 10 sporadic pituitary somatotroph adenoma patients and grouped according to the pathological type. Tandem mass tag (TMT)-based quantitative proteomic analysis was employed to analyze the proteomic differences between SG and DG tumors.

resultsIn total, 228 differentially expressed proteins were identified between SG adenomas and DG adenomas. They were enriched mainly in extracellular matrix (ECM)-receptor interaction, leukocyte transendothelial migration, arrhythmogenic right ventricular cardiomyopathy and DNA replication pathways. Protein-protein interaction (PPI) network analysis indicated that Cadherin-1 and Catenin beta-1 were the most important key proteins in the differences between SG and DG adenomas. Immunohistochemistry (IHC) confirmed the expression levels of the key proteins.

conclusionsThis study provides large-scale proteome molecular characteristics of distinct granulation subtypes of somatotroph adenomas. Compared with DG adenomas, The differential protein of SG adenomas mostly enrich in invasive and proliferative functions and pathways at the proteomic level. Cadherin-1 and Catenin beta-1 play key roles in the different biological characteristics of the two tumor subtypes.

Indexed as

AcromegalyAdenomaGrowth Hormone-Secreting Pituitary AdenomaPituitary NeoplasmsHumansProteomicsAcromegalyGHMolecular networkPituitary adenomaQuantitative proteomicsTandem mass tag (TMT)

Identifiers

PMID34043183

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.