Evidence map›Paper›PMID 34041585›Full record

ArticleMikrochimica acta2021

Voltammetric-based immunosensor for the detection of SARS-CoV-2 nucleocapsid antigen.

Shimaa Eissa, Hani A Alhadrami, Maha Al-Mozaini, Ahmed M Hassan, Mohammed Zourob

Open access · bronzeAbstract read
In one paragraph

Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.

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  17. Recent advances in point of care testing for COVID-19 detection.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Shimaa EissaDepartment of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia.
Hani A AlhadramiFaculty of Applied Medical Sciences, King Abdulaziz University, P. O. Box 80402, Jeddah, 21589, Kingdom of Saudi Arabia.
Maha Al-MozainiImmunocompromised Host Research, Department of infection and Immunity, King Faisal Specialist Hospital and Research Center, Zahrawi Street, Al Maather, Riyadh, 12713, Saudi Arabia.
Ahmed M HassanMolecular Diagnostic Lab, King Abdulaziz University Hospital, King Abdulaziz University, P. O. Box 80402, Jeddah, 21589, Saudi Arabia.
Mohammed ZourobDepartment of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia. mzourob@alfaisal.edu.ORCID http://orcid.org/0000-0003-2187-1430
Alfaisal University · SAKing Abdulaziz University · SAKing Faisal Specialist Hospital & Research Centre · SA

Funding

King Abdulaziz City for Science and Technology 5-20-01-023-0009
6 · The paper itself

Abstract

Since the COVID-19 disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) was declared a pandemic, it has spread rapidly, causing one of the most serious outbreaks in the last century. Reliable and rapid diagnostic tests for COVID-19 are crucial to control and manage the outbreak. Here, a label-free square wave voltammetry-based biosensing platform for the detection of SARS-CoV-2 in nasopharyngeal samples is reported. The sensor was constructed on screen-printed carbon electrodes coated with gold nanoparticles. The electrodes were functionalized using 11-mercaptoundecanoic acid (MUA) which was used for the immobilization of an antibody against SARS-CoV-2 nucleocapsid protein (N protein). The binding of the immunosensor with the N protein caused a change in the electrochemical signal. The detection was realised by measuring the change in reduction peak current of a redox couple using square wave voltammetry at 0.04 V versus Ag ref. electrode on the immunosensor upon binding with the N protein. The electrochemical immunosensor showed high sensitivity with a linear range from 1.0 pg.mL

Indexed as

Antibodies, ImmobilizedBiosensing TechniquesCarbonCoronavirus Nucleocapsid ProteinsCOVID-19COVID-19 TestingElectrochemical TechniquesElectrodesFatty AcidsGoldHumansImmunoassayLimit of DetectionMetal NanoparticlesNasopharynxPhosphoproteins11-mercaptoundecanoic acidAntibodies, ImmobilizedCarbonCoronavirus Nucleocapsid ProteinsFatty AcidsGoldnucleocapsid phosphoprotein, SARS-CoV-2PhosphoproteinsSulfhydryl CompoundsAntibodyBiosensorElectrochemical detectionImmunosensorNucleocapsid proteinVoltammetry

Identifiers

PMID34041585
PMCPMC8153846
OpenAlexW3165832267

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.