Evidence map›Paper›PMID 34041514›Full record

ArticleSoft matter2021

Confinement size determines the architecture of Ran-induced microtubule networks.

Ya Gai, Brian Cook, Sagar Setru, Howard A Stone, Sabine Petry

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Soft matter, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. How Microtubules Build the Spindle Branch by Branch.Annual review of cell and developmental biology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Ya GaiDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, USA. hastone@princeton.edu.ORCID http://orcid.org/0000-0003-2709-9576
Brian CookDepartment of Molecular Biology, Princeton University, Princeton, NJ, USA. spetry@princeton.edu.
Sagar SetruLewis-Sigler Institute of Integrative Genomics, Princeton University, Princeton, NJ, USA.
Howard A StoneDepartment of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, USA. hastone@princeton.edu.ORCID http://orcid.org/0000-0002-9670-0639
Sabine PetryDepartment of Molecular Biology, Princeton University, Princeton, NJ, USA. spetry@princeton.edu.ORCID http://orcid.org/0000-0002-8537-9763
Princeton University · USInstitute of Genomics and Integrative Biology · IN

Funding

Building the Chromosome Segregation Machinery from ScratchDP2GM123493 · NIGMS · PRINCETON UNIVERSITY · PI PETRY, SABINE · 2016 to 2016
$2.4M
NIGMS NIH HHS DP2 GM123493
6 · The paper itself

Abstract

The organization of microtubules (MTs) is critical for cells during interphase and mitosis. During mitotic spindle assembly, MTs are made and organized around chromosomes in a process regulated by RanGTP. The role of RanGTP has been explored in Xenopus egg extracts, which are not limited by a cell membrane. Here, we investigated whether cell-sized confinements affect the assembly of RanGTP-induced MT networks in Xenopus egg extracts. We used microfluidics to encapsulate extracts within monodisperse extract-in-oil droplets. Importantly, we find that the architecture of Ran-induced MT networks depends on the droplet diameter and the Ran concentration, and differs from structures formed in bulk extracts. Our results highlight that both MT nucleation and physical confinement play critical roles in determining the spatial organization of the MT cytoskeleton.

Indexed as

Spindle ApparatusXenopus ProteinsMicrotubule-Associated ProteinsMicrotubulesran GTP-Binding ProteinMicrotubule-Associated Proteinsran GTP-Binding ProteinXenopus Proteins

Identifiers

PMID34041514
PMCPMC8958645
OpenAlexW3161543763

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.