Evidence map›Paper›PMID 34041500›Full record

ArticleSTAR protocols2021

Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms.

Laura E McKnight, Johnathan G Crandall, Thomas B Bailey, Orion G B Banks, Kona N Orlandi, Vi N Truong, Drake A Donovan, Grace L Waddell, Elizabeth T Wiles, Scott D Hansen and 2 more

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

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  8. The RPD3L deacetylation complex is required for facultative heterochromatin repression inProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Laura E McKnightInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Johnathan G CrandallInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Thomas B BaileyInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Orion G B BanksInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Kona N OrlandiInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Vi N TruongInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Drake A DonovanInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Grace L WaddellInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Elizabeth T WilesInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Scott D HansenInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Eric U SelkerInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Jeffrey N McKnightInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
University of Oregon · US

Funding

MOLECULAR BIOLOGY AND BIOPHYSICST32GM007759 · NIGMS · UNIVERSITY OF OREGON · PI NOLEN, BRADLEY J · 1985 to 2021
$8.5M
Graduate Training in Genetics Administrative Supplement - Career Curriculum DevelopmentT32GM007413 · NIGMS · UNIVERSITY OF OREGON · PI GUILLEMIN, KAREN J · 1985 to 2022
$7.8M
Control and function of heterochromatin in Neurospora crassaR35GM127142 · NIGMS · UNIVERSITY OF OREGON · PI SELKER, ERIC U. · 2018 to 2022
$2.6M
Mechanisms and Consequences of Specific Chromatin Arrangements in vivoR01GM129242 · NIGMS · UNIVERSITY OF OREGON · PI MCKNIGHT, LAURA EMILY · 2018 to 2022
$1.5M
Control and Function of Histone H3 Lysine 27 Methylation in NeurosporaR01GM093061 · NIGMS · UNIVERSITY OF OREGON · PI SELKER, ERIC U. · 2012 to 2015
$1.1M
NIGMS NIH HHS R01 GM093061NIGMS NIH HHS R01 GM129242NIGMS NIH HHS R35 GM127142NIGMS NIH HHS T32 GM007413NIGMS NIH HHS T32 GM007759
6 · The paper itself

Abstract

MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a streamlined protocol for

Indexed as

NucleosomesAnimalsCell Culture TechniquesCells, CulturedDNADNA FootprintingGenomicsMicrococcal NucleaseSaccharomyces cerevisiaeSequence Analysis, DNADNAMicrococcal NucleaseNucleosomesGenomicsModel OrganismsMolecular BiologySequencing

Identifiers

PMID34041500
PMCPMC8141940
OpenAlexW3162700230

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.