ArticleChemical science2021
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Reversible, Chemically Gated FRET via Ligand-Activated Acceptors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A color-tailored fluorogenic sensor for hydrogen peroxide.Nature chemical biology · 2026Article
- Bathochromic Shift via C=O to C=S Substitution: A Far-Red Fluorogen for Multiplexed FLIM with FAST Fluorogen-Activating Protein.International journal of molecular sciences · 2025Article
- Fluorescence Lifetime-Based Separation of FAST-Labeled Cellular Compartment.Bio-protocol · 2025Article
- A twisted chromophore powers a turn-on fluorescent protein chloride sensor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes.Nature communications · 2025Article
- A tunable and versatile chemogenetic near-infrared fluorescent reporter.Nature communications · 2025Article
- A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.The Journal of cell biology · 2024Article
- Fluorescence lifetime multiplexing with fluorogen activating protein FAST variants.Communications biology · 2024Article
- A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST.International journal of molecular sciences · 2024Article
- Dynamic Structures of Bioactive Proteins as Determined by Nuclear Magnetic Resonance.International journal of molecular sciences · 2023Article
- A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.bioRxiv : the preprint server for biology · 2023Article
- Microbiome imaging goes à la carte: Incorporating click chemistry into the fluorescence-activating and absorption-shifting tag (FAST) imaging platform.bioRxiv : the preprint server for biology · 2023Article
- Beyond DNA: new probes for PAINT super-resolution microscopy.Chemical communications (Cambridge, England) · 2023Review
- Engineering fluorescent protein chromophores with an internal reference for high-fidelity ratiometric G4 imaging in living cells.Chemical science · 2023Article
- Multifunctional stimuli-responsive chemogenetic platform for conditional multicolor cell-selective labeling.Chemical science · 2022Article
- Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2.International journal of molecular sciences · 2022Article
- Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore.Communications biology · 2022Article
- Recent Advancements in Tracking Bacterial Effector Protein Translocation.Microorganisms · 2022Review
- Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.Nature communications · 2021Article
Corrections and comments
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Authors and funding
19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.