Evidence map›Paper›PMID 34040392›Full record

ArticleOncoTargets and therapy2021

Circular RNA circWDR27 Promotes Papillary Thyroid Cancer Progression by Regulating miR-215-5p/TRIM44 Axis.

Weilan Wang, Chengmin Huang, Peng Luo, Jiang Yao, Jie Li, Wenxia Wang, Fengqin Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in OncoTargets and therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Weilan Wang *Department of General Surgery, Changxing People's Hospital, The Second Affiliated Hospital of Zhejiang University School of Medical Changxing Campus, Changxing, Zhejiang, People's Republic of China.
Chengmin Huang *Department of General Surgery, Changxing People's Hospital, The Second Affiliated Hospital of Zhejiang University School of Medical Changxing Campus, Changxing, Zhejiang, People's Republic of China.
Peng LuoDepartment of Medical Research, Shanghai Topgen Biomedical Technology Co., Ltd., Shanghai, People's Republic of China.
Jiang YaoDepartment of General Surgery, Changxing People's Hospital, The Second Affiliated Hospital of Zhejiang University School of Medical Changxing Campus, Changxing, Zhejiang, People's Republic of China.
Jie LiDepartment of General Surgery, Changxing People's Hospital, The Second Affiliated Hospital of Zhejiang University School of Medical Changxing Campus, Changxing, Zhejiang, People's Republic of China.
Wenxia WangDepartment of General Surgery, Changxing People's Hospital, The Second Affiliated Hospital of Zhejiang University School of Medical Changxing Campus, Changxing, Zhejiang, People's Republic of China.
Fengqin LiuDepartment of Ultrasound, Changxing People's Hospital, The Second Affiliated Hospital of Zhejiang University School of Medical Changxing Campus, Changxing, Zhejiang, People's Republic of China.
Second Affiliated Hospital of Zhejiang University · CNHealth Biomed (China) · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study was to explore the biological roles and underlying mechanism of circRNA WD repeat domain 27 (circWDR27).

methodsThe expression of circWDR27, microRNA-215-5p (miR-215-5p) and tripartite motif containing 44 (TRIM44) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays were employed to detect cell proliferation. Flow cytometry was used to determine cell apoptosis and cell cycle distribution. Cell migration and invasion abilities were examined by wound healing and transwell assays. The protein levels of matrix metalloproteinase 2 (MMP2), MMP9 and TRIM44 were analyzed by Western blot assay. The relationship between miR-215-5p and circWDR27 or TRIM44 was predicted by bioinformatics tools and confirmed using dual-luciferase reporter assay. Mouse xenograft model was established to examine the role of circWDR27 in vivo.

resultsCircWDR27 and TRIM44 were highly expressed while miR-215-5p was lowly expressed in PTC tissues and cells. Knockdown of circWDR27 suppressed cell proliferation and metastasis and induced cell cycle arrest and apoptosis in PTC cells. Moreover, miR-215-5p was a direct target of circWDR27, and its inhibition reversed the suppressive effect of circWDR27 knockdown on PTC cell progression. In addition, miR-215-5p directly targeted TRIM44, and miR-215-5p exerted its anti-cancer role in PTC cells by targeting TRIM44. Furthermore, circWDR27 positively regulated TRIM44 expression by sponging miR-215-5p. Importantly, knockdown of circWDR27 suppressed tumor growth in vivo by upregulating miR-215-5p and downregulating TRIM44.

conclusionCircWDR27 accelerates PTC progression via regulating miR-215-5p/TRIM44 axis, providing a potential therapeutic target for PTC.

Indexed as

circWDR27miR-215–5ppapillary thyroid cancerTRIM44

Identifiers

PMID34040392
PMCPMC8141407
OpenAlexW3163426499

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.