Evidence map›Paper›PMID 34038755›Full record

ArticleBiochemical and biophysical research communications2021

A novel XBP1 variant is highly enriched in cancer tissues and is specifically required for cancer cell survival.

Yongwang Zhong, Wenjing Yan, Jingjing Ruan, Mike Fang, Rena G Lapidus, Shaojun Du, Shengyun Fang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Yongwang ZhongCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Wenjing YanCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Jingjing RuanCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Department of Pulmonary Medicine, Anhui Medical University First Affiliated Hospital, Hefei, Anhui, 230032, China.
Mike FangCase Western Reserve University, Cleveland, OH, USA.
Rena G LapidusTranslational Laboratory Shared Service, UM Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Shaojun DuDepartment of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Shengyun FangCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Program in Oncology, UM Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD, 21201, USA. Electronic address: sfang@som.umaryland.edu.
University of Maryland, Baltimore · USCase Western Reserve University · US

Funding

Pharmacologic enhancement of UBA1 activity in models of VEXAS syndromeU01AR081599 · NIAMS · UNIVERSITY OF MARYLAND BALTIMORE · PI Shengyun Fang · 2022 to 2026
$1.9M
High-Content Screening to Identify Small Molecules for Refolding SOD1 MutantsU01GM117175 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI FANG, SHENGYUN · 2017 to 2019
$1.1M
NIAMS NIH HHS U01 AR081599NIGMS NIH HHS U01 GM117175
6 · The paper itself

Abstract

XBP1 is a basic leucine zipper (bZIP) transcription factor and a key mediator of the endoplasmic reticulum (ER) stress-activated unfolded protein response (UPR). XBP1-mediated transcription facilitates cell adaptation to ER stress and also promotes tumor progression, while suppressing anti-tumor immunity. Here we report a novel XBP1 variant, namely XBP1 variant 1 (XBP1v1, Xv1 for short), that is specifically required for survival of cancer cells. Xv1 contains a cryptic first exon that is conserved only in humans and great apes. Comparing to XBP1, Xv1 encodes a protein with a different N-terminal sequence containing 25 amino acids. Analysis of RNAseq database reveals that Xv1 is broadly expressed across cancer types but almost none in normal tissues. Elevated Xv1 expression is associated with poor survival of patients with several types of cancer. Knockdown of Xv1 induces death of multiple cancer cell lines but has little effect on non-cancerous cells in vitro. Moreover, knockdown of Xv1 also inhibits growth of a xenograft breast tumor in mice. Together, our results indicate that Xv1 is essential for survival of cancer cells.

Indexed as

Genetic VariationAnimalsCell Line, TumorCell SurvivalFemaleGene Expression Regulation, NeoplasticHumansMiceMice, NudeNeoplasmsRNA, MessengerX-Box Binding Protein 1Xenograft Model Antitumor AssaysRNA, MessengerX-Box Binding Protein 1Cancer cell survivalXBP1XBP1v1Xv1

Identifiers

PMID34038755
PMCPMC8206033
OpenAlexW3165219089

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.