Evidence map›Paper›PMID 34038205›Full record

ArticleThe Journal of international medical research2021

Possible involvement of the Hedgehog and PDPK1-Akt pathways in the growth and migration of small-cell lung cancer.

Naiwang Tang, Bin Hu, Yin Zhang, Zhiwei Chen, Ronghuan Yu

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PubMed Full text at PMC Publisher
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Article in The Journal of international medical research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Naiwang TangDepartment of Respiratory Medicine, Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University, Shanghai, China.
Bin HuDepartment of Respiratory Medicine, Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University, Shanghai, China.
Yin ZhangDepartment of Respiratory Rehabilitation, Shanghai Fourth Rehabilitation Hospital, Shanghai, China.
Zhiwei ChenShanghai Lung Tumor Clinical Medical Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.
Ronghuan YuDepartment of Respiratory Medicine, Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0003-1782-3823

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSmall-cell lung cancer (SCLC) accounts for approximately 15% to 20% of all lung cancers, and it is the leading cause of tumor-related deaths globally. This study explored the molecular mechanisms underlying the development of SCLC.

methodsThe correlations of phosphoinositide-dependent kinase-1 (PDPK1), p-Akt, and Hedgehog expression with patient characteristics were analyzed using SCLC specimens, and their expression was measured in BEAS-2B cells (control) and the SCLC cell lines H82, H69, H446, H146, and H526. Transfection experiments were performed to inhibit or activate gene expression in cells. We then measured the proliferation and migration of H146 cells.

resultsPDPK1, p-Akt, and Hedgehog expression was significantly higher in SCLC tissues, and their expression was correlated with patient characteristics. p-Akt expression was significantly correlated with Hedgehog expression. In H146 cells, PDPK1 and p-Akt were significantly upregulated. Silencing of PDPK1 or Akt and inhibition of Hedgehog significantly inhibited the proliferation and migration of H146 cells. PDPK1 and Akt affected Hedgehog expression, but Hedgehog did not affect PDPK1 or p-Akt expression.

conclusionsThe interaction between the PDPK1-Akt pathway and the Hedgehog pathway influences the prognosis, growth, and migration of SCLC.

Indexed as

Hedgehog ProteinsLung Neoplasms1-Phosphatidylinositol 4-Kinase3-Phosphoinositide-Dependent Protein KinasesCell Line, TumorCell ProliferationHumansProto-Oncogene Proteins c-akt1-Phosphatidylinositol 4-Kinase3-Phosphoinositide-Dependent Protein KinasesHedgehog ProteinsPDPK1 protein, humanProto-Oncogene Proteins c-aktAktHedgehoginvasionmigrationPhosphoinositide-dependent kinase-1proliferationsmall-cell lung cancer

Identifiers

PMID34038205
PMCPMC8161885

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.