Evidence map›Paper›PMID 34038030›Full record

ArticleCurrent protocols2021

ImageJ and CellProfiler: Complements in Open-Source Bioimage Analysis.

Ellen T A Dobson, Beth Cimini, Anna H Klemm, Carolina Wählby, Anne E Carpenter, Kevin W Eliceiri

Abstract read
In one paragraph

Article in Current protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ellen T A DobsonLaboratory for Optical and Computational Instrumentation (LOCI), Center for Quantitative Cell Imaging, University of Wisconsin at Madison, Madison, Wisconsin.
Beth CiminiImaging Platform, Broad Institute of Harvard and MIT, Cambridge, Massachusetts.
Anna H KlemmScience for Life Laboratory BioImage Informatics Facility and Department of Information Technology, Uppsala University, Uppsala, Sweden.
Carolina WählbyScience for Life Laboratory BioImage Informatics Facility and Department of Information Technology, Uppsala University, Uppsala, Sweden.
Anne E CarpenterImaging Platform, Broad Institute of Harvard and MIT, Cambridge, Massachusetts.
Kevin W EliceiriLaboratory for Optical and Computational Instrumentation (LOCI), Center for Quantitative Cell Imaging, University of Wisconsin at Madison, Madison, Wisconsin.

Funding

Center for Open Bioimage AnalysisP41GM135019 · NIGMS · BROAD INSTITUTE, INC. · PI CARPENTER, ANNE E., CIMINI, BETH · 2020 to 2024
$6.9M
NIGMS NIH HHS P41 GM135019NIH HHS GM135019
6 · The paper itself

Abstract

ImageJ and CellProfiler have long been leading open-source platforms in the field of bioimage analysis. ImageJ's traditional strength is in single-image processing and investigation, while CellProfiler is designed for building large-scale, modular analysis pipelines. Although many image analysis problems can be well solved with one or the other, using these two platforms together in a single workflow can be powerful. Here, we share two pipelines demonstrating mechanisms for productively and conveniently integrating ImageJ and CellProfiler for (1) studying cell morphology and migration via tracking, and (2) advanced stitching techniques for handling large, tiled image sets to improve segmentation. No single platform can provide all the key and most efficient functionality needed for all studies. While both programs can be and are often used separately, these pipelines demonstrate the benefits of using them together for image analysis workflows. ImageJ and CellProfiler are both committed to interoperability between their platforms, with ongoing development to improve how both are leveraged from the other. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Studying cell morphology and cell migration in time-lapse datasets using TrackMate (Fiji) and CellProfiler Basic Protocol 2: Creating whole plate montages to easily assess adaptability of segmentation parameters.

Indexed as

Image Processing, Computer-AssistedSoftwareAnimalsCell CountCell MovementCell ShapeHumansTime-Lapse ImagingCellProfilercell trackingImageJimage stitchingsegmentation

Identifiers

PMID34038030
PMCPMC8450896

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.