ArticleAmerican journal of physiology. Lung cellular and molecular physiology2021
Passive siRNA transfection method for gene knockdown in air-liquid interface airway epithelial cell cultures.
Article in American journal of physiology. Lung cellular and molecular physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 17 citations in OpenAlex.
- Polymeric nanoparticle-mediated siRNA delivery into primary human bronchial airway cells.American journal of physiology. Cell physiology · 2026Article
- Imaging and safety profiling of inhaled siRNA RyR2 in human respiratory models.Archives of toxicology · 2026Article
- LARS promotes hepatocellular carcinoma progression via the PI3K/AKT/mTOR pathway and interaction with RPS5, and serves as a prognostic biomarker.Molecular medicine (Cambridge, Mass.) · 2026Article
- Inhibition of Airway Epithelial CTSC Attenuates Type 2 Inflammation: Implications for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps.Journal of inflammation research · 2026Article
- Investigating the Role of KNG1 in Traumatic Brain Injury.Journal of inflammation research · 2026Article
- The extracellular matrix protein periostin is required for wound repair in primary human airway epithelia.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Nasal Mucociliary Epithelial Cell Culture Models for Studying Viral Infections.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Akt activator SC79 stimulates antibacterial nitric oxide generation in human nasal epithelial cells in vitro.International forum of allergy & rhinology · 2024Article
- Modeling the Human Alveolar Epithelium: Promises and Challenges.American journal of respiratory cell and molecular biology · 2024Article
- MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability.JCI insight · 2023Article
- Loss of CFTR function is associated with reduced bitter taste receptor-stimulated nitric oxide innate immune responses in nasal epithelial cells and macrophages.Frontiers in immunology · 2023Article
- Genomic evidence for dysregulated glutamine metabolism in the asthmatic airway epithelium.Clinical and translational allergy · 2022Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
Differentiation of human bronchial epithelial cells (HBEs) in air-liquid interface (ALI) cultures recapitulates organotypic modeling of the in vivo environment. Although ALI cultures are invaluable for studying the respiratory epithelial barrier, loss-of-function studies are limited by potentially cytotoxic reagents in classical transfection methods, the length of the differentiation protocol, and the number of primary epithelial cell passages. Here, we present the efficacy and use of a simple method for small interfering RNA (siRNA) transfection of normal HBEs (NHBEs) in ALI cultures that does not require potentially cytotoxic transfection reagents and does not detrimentally alter the physiology or morphology of NHBEs during the differentiation process. This transfection protocol introduces a reproducible and efficient method for loss-of-function studies in HBE ALI cultures that can be leveraged for modeling the respiratory system and airway diseases.
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