Evidence map›Paper›PMID 34033088›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.

Nune Darbinian, Armine Darbinyan, Kamel Khalili, Shohreh Amini

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
10.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Frontiers in neuroscience · 2023
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Nune DarbinianDepartment of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. nsarkiss@temple.edu.
Armine DarbinyanDepartment of Pathology, Yale University School of Medicine, New Haven, CT, USA.
Kamel KhaliliDepartment of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Shohreh AminiDepartment of Biology, College of Science & Technology, Temple University, Philadelphia, PA, USA.
Temple University · USYale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The lack of a convenient in vitro human neuronal model to study alcohol-induced neurodegenerative diseases, such as fetal alcohol syndrome (FAS), prompted us to develop human neuronal culture and in vitro human FAS model by incubating cells with physiologically relevant EtOH concentration (50 mM). Here, we describe the detailed method of isolation of human neuronal culture, and ability to analyze neurites extension using Sholl assay. We utilized highly efficient transfection method of neuronal cells to study morphology of neurons with or without EtOH treatment.

Indexed as

Biological AssayAnimalsBrainCells, CulturedCell SeparationEthanolFetal Alcohol Spectrum DisordersGestational AgeHumansNeuronal OutgrowthNeuronsPrimary Cell CultureTransfectionEthanolAlcoholFASHuman neuronal cellsSholl assay

Identifiers

PMID34033088
OpenAlexW3164819765

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.