Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.

Nune Darbinian, Armine Darbinyan, Nana Merabova, Shohreh Amini

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

1.1field-weighted citation impact, top 21% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

Frontiers in neuroscience · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Nune DarbinianDepartment of Neurology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. nsarkiss@temple.edu.

Armine DarbinyanDepartment of Pathology, Yale University School of Medicine, New Haven, CT, USA.

Nana MerabovaShriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA.

Shohreh AminiDepartment of Biology, College of Science & Technology, Temple University, Philadelphia, PA, USA.

Temple University · USTemple University Hospital · USYale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studies of blood-brain barrier (BBB) require developing of a novel and convenient in vitro endothelial cell model. We isolated primary human and rodent brain microvascular endothelial cells and developed methods for culturing, characterization, and high-efficiency transfection of endothelial cells. Here, we describe the improved methods to obtain in vitro human and rodent BBB models to study expression of endogenous and exogenous genes of interest.

Indexed as

Cell SeparationTransfectionAnimalsBlood-Brain BarrierBrainCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedEndothelial CellsFetusGestational AgeHumansMiceMicrovesselsRatsDifferentiationHuman primary endothelial cellsMouse endothelial cellsNucleofectionRat primary endothelial culture

Identifiers

PMID34033087

DOI10.1016/j.antiviral.2007.11.006

OpenAlexW3164652720

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.