ArticleeLife2021
Optogenetic strategies for high-efficiency all-optical interrogation using blue-light-sensitive opsins.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- The Importance of Synchrony in the Neural Control of Movement.bioRxiv : the preprint server for biology · 2026Article
- PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.Nature methods · 2026Article
- The parafascicular nucleus of the thalamus orchestrates coordinated skeletomotor, autonomic, and aversive state transitions.bioRxiv : the preprint server for biology · 2026Article
- Active pixel power control for crosstalk-free all-optical neural interrogation.Nature communications · 2026Article
- Technical development of two-photon optogenetic stimulation and its potential application to brain-machine interfaces.Neurophotonics · 2026Review
- Desensitization of opsin responses during all-optical interrogation depends on imaging parameters.Neurophotonics · 2026Article
- Red-shifted excitation enhances the sensitivity of red genetically encoded CaJournal of biomedical optics · 2025Article
- A genetically defined pontine nucleus essential for ingestion in mice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- From microelectrode arrays to all-optical and multimodal neural interfaces: emerging platforms for spatiotemporal interrogation ofFrontiers in synaptic neuroscience · 2025Review
- Contribution of optical resolution to the spatial precision of two-photon optogenetic photostimulationNeurophotonics · 2024Article
- Flexible electronic-photonic 3D integration from ultrathin polymer chiplets.Npj flexible electronics · 2024Article
- DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples.Biomedical optics express · 2023Article
- Ultrafast light targeting for high-throughput precise control of neuronal networks.Nature communications · 2023Article
- Determinants of functional synaptic connectivity among amygdala-projecting prefrontal cortical neurons in male mice.Nature communications · 2023Article
- Article
- All-optical interrogation of neural circuits in behaving mice.Nature protocols · 2022Review
- Engineering rhodopsins' activation spectra using a FRET-based approach.Biophysical journal · 2022Article
- A deep-learning approach for online cell identification and trace extraction in functional two-photon calcium imaging.Nature communications · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
All-optical methods for imaging and manipulating brain networks with high spatial resolution are fundamental to study how neuronal ensembles drive behavior. Stimulation of neuronal ensembles using two-photon holographic techniques requires high-sensitivity actuators to avoid photodamage and heating. Moreover, two-photon-excitable opsins should be insensitive to light at wavelengths used for imaging. To achieve this goal, we developed a novel soma-targeted variant of the large-conductance blue-light-sensitive opsin CoChR (stCoChR). In the mouse cortex in vivo, we combined holographic two-photon stimulation of stCoChR with an amplified laser tuned at the opsin absorption peak and two-photon imaging of the red-shifted indicator jRCaMP1a. Compared to previously characterized blue-light-sensitive soma-targeted opsins in vivo, stCoChR allowed neuronal stimulation with more than 10-fold lower average power and no spectral crosstalk. The combination of stCoChR, tuned amplified laser stimulation, and red-shifted functional indicators promises to be a powerful tool for large-scale interrogation of neural networks in the intact brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.